Present address: Department of Pathology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Isolation and expression of a sterol carrier protein-2 gene from the yellow fever mosquito, Aedes aegypti
Article first published online: 22 JAN 2003
Insect Molecular Biology
Volume 12, Issue 1, pages 51–60, February 2003
How to Cite
Krebs, K. C. and Lan, Q. (2003), Isolation and expression of a sterol carrier protein-2 gene from the yellow fever mosquito, Aedes aegypti. Insect Molecular Biology, 12: 51–60. doi: 10.1046/j.1365-2583.2003.00386.x
- Issue published online: 22 JAN 2003
- Article first published online: 22 JAN 2003
- Received 15 May 2002; accepted after revision 26 September 2002.
- sterol carrier protein-2;
- gene expression;
Trafficking of cholesterol in insects is a very important process due to the fact that insects depend on dietary cholesterol to fulfil their physiological needs. We identified a putative mosquito sterol carrier protein-2 (SCP-2) cDNA from fourth instar subtracted cDNA library. The AeSCP-2 protein has high degree homology in the sterol transfer domain to both rat and human SCP-2. Transcripts of AeSCP-2 in fourth instars were detected strongly in the midgut, and weakly in the head and hindgut. In the early pupae, AeSCP-2 transcription was observed in the thorax, head and body wall of abdomen, but not in the gut.
The interaction of mosquito sterol carrier protein-2 (AeSCP-2) with cholesterol was examined. The Kd of purified recombinant AeSCP-2 to cholesterol was 5.6 ± 0.6 × 10−9 m using radiolabelled cholesterol-binding assay. The results suggest that AeSCP-2 has high affinity to cholesterol and may function as a carrier protein in mosquitoes.