Characterization of JNK-like protein derived from a mosquito cell line, C6/36
Article first published online: 22 JAN 2003
Insect Molecular Biology
Volume 12, Issue 1, pages 61–66, February 2003
How to Cite
Mizutani, T., Kobayashi, M., Eshita, Y., Inanami, O., Yamamori, T., Goto, A., Ako, Y., Miyoshi, H., Miyamoto, H., Kariwa, H., Kuwabara, M. and Takashima, I. (2003), Characterization of JNK-like protein derived from a mosquito cell line, C6/36. Insect Molecular Biology, 12: 61–66. doi: 10.1046/j.1365-2583.2003.00387.x
- Issue published online: 22 JAN 2003
- Article first published online: 22 JAN 2003
- Received 3 July 2002; accepted after revision 30 September 2002.
- Aedes albopictus;
- mitogen-activated protein kinase;
- c-Jun amino-terminal kinase;
When Western blot analysis of heat-killed bacteria- and lipopolysaccharide (LPS)-treated Aedes albopictus mosquito cell line C6/36 was performed using antiphospholyrated c-Jun amino-terminal kinase (JNK) antibodies, approximately 46 kDa protein was clearly detected with a peak around 30 min. After the C6/36 cells were incubated at 45 °C in order to induce apoptosis, the 46 kDa protein continued to be detected for at least 3 h. The internalization of fluorescein-labelled bacteria was inhibited by a JNK-specific inhibitor SP600125, suggesting that phagocytosis involves the JNK signalling pathway in mosquito cells. Based on these results, we found one candidate for the nucleotide sequence of JNK (Ae-JNK) from the C6/36 cells. This study is the first report regarding the mitogen-activated protein kinase (MAPK) of mosquito.