Chemosensory proteins of Locusta migratoria
Version of Record online: 24 MAR 2003
Insect Molecular Biology
Volume 12, Issue 2, pages 125–134, April 2003
How to Cite
Ban, L., Scaloni, A., Brandazza, A., Angeli, S., Zhang, L., Yan, Y. and Pelosi, P. (2003), Chemosensory proteins of Locusta migratoria. Insect Molecular Biology, 12: 125–134. doi: 10.1046/j.1365-2583.2003.00394.x
- Issue online: 24 MAR 2003
- Version of Record online: 24 MAR 2003
- Received 16 September 2002; accepted after revision 22 November 2002.
- chemosensory proteins;
- chemical communication;
- fluorescence binding assay;
- Locusta migratoria
Two different classes of chemosensory proteins (CSPs) in Locusta migratoria have been identified on the basis of the molecular cloning of a series of different cDNAs from the antennae of this insect. Several CSP isoforms have been purified and biochemically characterized from antennal and wing extracts, some of them corresponding to expression products predicted for the identified cDNAs. In wings, the nature of the main endogenous ligand binding to these proteins was determined as oleoamide by a gas chromatography–mass spectrometric approach. One of these isoforms has been expressed in a bacterial system with high yield and used in a fluorescent binding assay. Competitive binding experiments have indicated the presence of long-chain compounds among the best ligands.