Note: These authors contributed equally to this study.
piggyBac-mediated germline transformation in the beetle Tribolium castaneum
Article first published online: 15 SEP 2003
Insect Molecular Biology
Volume 12, Issue 5, pages 433–440, October 2003
How to Cite
Lorenzen, M. D., Berghammer, A. J., Brown, S. J., Denell, R. E., Klingler, M. and Beeman, R. W. (2003), piggyBac-mediated germline transformation in the beetle Tribolium castaneum. Insect Molecular Biology, 12: 433–440. doi: 10.1046/j.1365-2583.2003.00427.x
- Issue published online: 15 SEP 2003
- Article first published online: 15 SEP 2003
- Received 27 January 2003; accepted after revision 9 May 2003.
- germline transformation;
- enhancer trap;
- Tribolium castaneum
The lepidopteran transposable element piggyBac can mediate germline insertions in at least four insect orders. It therefore shows promise as a broad-spectrum transformation vector, but applications such as enhancer trapping and transposon-tag mutagenesis are still lacking. We created, cloned, sequenced and genetically mapped a set of piggyBac insertions in the red flour beetle, Tribolium castaneum. Transpositions were precise, and specifically targeted the canonical TTAA recognition sequence. We detected several novel reporter-expression domains, indicating that piggyBac could be used to identify enhancer regions. We also demonstrated that a primary insertion of a non-autonomous element can be efficiently remobilized to non-homologous chromosomes by injection of an immobile helper element into embryos harbouring the primary insertion. These developments suggest potential for more sophisticated methods of piggyBac-mediated genome manipulation.