Immunolocalization of bone extracellular matrix proteins (type I collagen, osteonectin and bone sialoprotein) in human dental pulp and cultured pulp cells
Article first published online: 11 JUN 2003
International Endodontic Journal
Volume 36, Issue 6, pages 404–410, June 2003
How to Cite
Garcia, J. M. Q., Martins, M. D., Jaeger, R. G. and Marques, M. M. (2003), Immunolocalization of bone extracellular matrix proteins (type I collagen, osteonectin and bone sialoprotein) in human dental pulp and cultured pulp cells. International Endodontic Journal, 36: 404–410. doi: 10.1046/j.1365-2591.2003.00669.x
- Issue published online: 11 JUN 2003
- Article first published online: 11 JUN 2003
- Received 17 May 2002; accepted 11 February 2003
- bone sialoprotein;
- cell culture;
- dental pulp;
- type I collagen
Aim To simultaneously analyse the expression of type I collagen, osteonectin and bone sialoprotein (BSP) in human dental pulp of different ages.
Methodology Cultured dental pulp fibroblasts (FP1 cell line), pulps from dental germs with incomplete root formation (n = 4) and pulps of erupted teeth with total root formation (n = 4) were used. Bone proteins were searched by immunohistochemistry and immunofluorescence using polyclonal antibodies and compared among the three groups assessed.
Results Immunohistochemistry detected the three proteins in dental pulp tissue, as it labelled extracellular matrix, predentine and odontoblasts. The BSP label was weaker, when compared to both type I collagen and osteonectin. The presence of type I collagen was more evident in pulps from erupted teeth, when compared to germ dental pulps. On the other hand, a strong expression of osteonectin in germ dental pulps was observed.
Conclusions Regardless of the degree of maturation, dental pulps present type I collagen, osteonectin and BSP in the extracellular matrix (ECM) and in the odontoblastic layer. Thus, the results suggest that these proteins are related to the production and mineralization of dentine.