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Dr M.-M. Lecadet, Laboratoire des Bactéries et Champignons Entomopathogènes, Institut Pasteur28, rue du Dr Roux, 75724 Paris Cedex 15, France.
The classification of Bacillus thuringiensis strains has been revised and updated based on flagellar antigens which have been in use for many years. Sixty-nine serotypes and 13 sub-antigenic groups have now been identified, giving 82 serovars among the 3500 B. thuringiensis isolates of the IEBC Collection. The number of serovars has gradually increased with the total number of strains. The biochemical characters used have also been investigated and their value assessed for identification of B. thuringiensis at the subspecies level. A crystal analysis was carried out in terms of morphology, δ-endotoxin profiles and larvicidal activity for the newly identified serovars. It was found that atypical crystals, some with novel components, are becoming more common. No insect susceptible to these serovars has been discovered among known target species. The number of cross-reacting H-antigens among B. cereus strains is increasing and may be of biological significance.
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The discovery of Bacillus thuringiensis strains with activity against certain species of Diptera (Goldberg & Margalit 1977; de Barjac 1978) and Coleoptera (Krieg et al. 1983) suggested possible new insecticidal treatments. This led to considerable attention being focused on this entomopathogenic bacterium, the activity spectrum of which was initially thought to be limited to insects in the order Lepidoptera. In the last 10 years there has been a continuous worldwide search for natural isolates with activity against economically important target insects (Martin & Travers 1989; Bernhard et al. 1997; Chaufaux et al. 1997).
Today, several tens of thousands of isolates, probably more than 50 000 (Sanchis et al. 1996) obtained from numerous screening procedures (Kalfon & de Barjac 1985; Ohba 1996), are distributed among various private and public collections, and are considered to be potential ‘reservoirs’ for novel toxins.
There are several reasons for the increased interest in B. thuringiensis. For example, emergence of insect populations resistant to many chemicals has been rapid and there is a fear that resistance to the widely used B. thuringiensis or B. sphaericus toxins may develop. The surprising diversity of B. thuringiensisδ-endotoxin genes (Höfte & Whiteley 1989; Lereclus et al. 1993), and evidence of their possible multiplicity within individual strains (Sanchis et al. 1988; Delécluse et al. 1989), make tentative classification based on pathotypes impossible. Thus, it has become increasingly necessary to have simple and reliable tools for classifying B. thuringiensis strains according to significant criteria.
Of the phenotypic methods used, H-serotyping and biochemical characters have both contributed to the establishment of a useful classification of B. thuringiensis isolates. The differentiation of these strains into serovarieties was developed on the basis of flagellar antigens by de Barjac & Bonnefoi (1962), and has been used ever since (de Barjac & Frachon 1990). New H-serotypes are numbered and registered at the International Entomopathogenic Bacillus Centre (IEBC) Collection (Burges et al. 1982) at Institut Pasteur, Paris, France. However, it is necessary to know whether such a system is still representative of the diversity of B. thuringiensis and whether it is relevant given the genetic exchanges that are known to occur between subspecies and with the closely related species, B. cereus.
This work updates the H-classification for which the number of serotypes has steadily increased. It also reports on the present status of biochemical characters. The limitations of these methods were considered and compared with other approaches, including molecular methods, in terms of taxonomic value.
Materials and methods
Origin of the strains
The B. thuringiensis strains included in this study were sent for identification to the IEBC (Unité des Bactéries Entomopathogènes at Institut Pasteur, France), a WHO Collaborating Centre. These strains, obtained from many research groups throughout the world (more than 22 countries), constitute the basis of the IEBC Collection registered under No. 590 in the World Data Centre on Microorganisms (Japan). They are listed in a catalogue, which is updated every 2 years.
H-serotyping and designation of new serotypes
Serotype was determined by tube agglutination tests, using diluted H-specific antisera, as previously described by de Barjac (1981) and recently reviewed by Thiéry & Frachon (1997). Reference H-antisera, representative of the various serovars, were used at dilutions of 1/200–1/25 600, to determine the agglutination titre defined as the lowest dilution that agglutinated the antigenic suspension tested. A modified protocol from Thiéry and Frachon now uses the Micronic® polycarbonate 96 tubes in plastic Micronic® boxes (Polylabo, France) to perform the preliminary determination of antigenic-antisera agglutination (antisera dilutions 1/100–1/400). This gains time and sera supply. Depending on this first result, titration is performed until dilution 1/25 600 of the reacted antisera is reached. Any B. thuringiensis strain not detected by any of the reference antisera available at the time is considered to be a potential new serotype. An antiserum directed against an antigenic suspension of such a strain was prepared and tested against H-antigens of all known serotypes to test whether or not they were agglutinated by the new antiserum. If a cross-reaction with other antigenic factors occurred, the antiserum saturation technique was used (de Barjac 1981) to identify possible antigenic subfactors determining distinct serovars within a known serotype.
A micromethod performed in 96-well microplates has recently been described (Laurent et al. 1996). This reduces the amount of serum required and saves time. The titration value was recorded using a magnifying glass or a Multi-Skan 352 MS apparatus (Life Sciences International; Labsystems OY, Finland) with a system to detect the wells in which agglutination took place.
Determination of biochemical characters
Standard biochemical tests are usually performed using classical methods as described by Sneath (1986) in Bergey's Manual and recorded by Smibert & Krieg (1994). During recent years, micromethods derived from the biochemical identification galleries have been introduced, especially regarding sugar utilization. The API identification systems for Bacillus sp. (API 20E + 50CHB; BioMerieux, Marcy l’Etoile, France), as reported by Logan & Berkeley (1984), are progressively replacing the classical methods that are still used as an alternative when necessary.
Presence and morphology of crystals
Sporulating cultures of the B. thuringiensis reference strains were produced in the standard B. thuringiensis medium (UG) containing Bactopeptone (7 g l−1), glucose and salts as previously reported (Lecadet & Dedonder 1971). The presence of crystals, and their morphology, was recorded during sporulation, before and after cell lysis after 24 and 48 h, by direct examination of these cultures under the phase contrast microscope (×1000), and confirmed by staining with Coomassie Brilliant Blue (0·25% solution in 50% ethanol and 7% acetic acid) as described by Sharif & Alaeddinoglu (1988) with the following modification: before coloration, bacterial smears on slides were washed with a 50/50 mixture of ethanol and acetone (Frachon unpublished). This staining procedure has proved very useful in many cases for confirming the presence of a parasporal inclusion.
The protein profiles of crystal components from the B. thuringiensis reference strains were determined by SDS-PAGE analysis, as described by Laemmli (1970), using 10% or 12·5% acrylamide separating gels. Samples (5–15 μg) of washed spore-crystal mixtures or purified crystals, prepared as described by Thomas & Ellar (1983), were placed in 2× concentrated sample buffer and heated at 100 °C for 10 min, as previously described (Lecadet et al. 1992) and loaded onto the gel immediately before electrophoresis.
Updating the serological classification
After more than three decades, classification of B. thuringiensis strains according to H-serotypes is still an efficient way of classifying strains on the basis of stable and specific characters, thereby preventing confusion with the thousands of isolates available worldwide.
The present state of this classification is shown in Table 1. By the end of 1998, 69 serotypes and 82 serovars were recorded and named. A previous classification status published by de Barjac & Frachon (1990) listed 27 serotypes and 34 serovars. Thus, 42 serotypes, or 48 serovars, were identified during this period, indicating a large increase.
Table 1. Classification of Bacillus thuringiensis strains according to the H serotype
Table 2 shows changes in the number of serovars relative to the total number of strains identified at the IEBC collection. Despite rapid increases since 1992, it is clear that the ratio has remained almost constant over the years, with a value of 2–2·5%. Therefore, the number of serotypes or serovars increases with the total number of isolates, leading to a relatively steady increase in the number of new H-antigenic structures. This suggests the stable development of these characters, resulting in a useful phenotypic classification which provides guidelines for the thousands of isolates in worldwide collections (Table 1). The new serovars were identified among strains from at least 22 countries: 11 of the serovars originated from Spain and the Azores, seven were from China, six from South Korea and three from South America (Brazil and Argentina). Many studies on serovar identification and preliminary characterization are now available or in progress.
Table 2. Changes in the number of serovars identified with the number of strains evaluated
The traditional phenotypic tests were long considered to be the key for the formal description of taxa at the species or subspecies level (Vandamme et al. 1996). However, de Barjac & Frachon (1990) showed with the 27 H-serotypes and the 34 serovars known at that time, that “most serovars cannot be distinguished by biochemical characters”. Therefore, how useful are biochemical characters for B. thuringiensis strain identification, given the large number of serovars identified? The use of miniaturized systems such as the commercially available API galleries, complemented by classical methods when necessary, is an effective way of comparing large numbers of strains, given the large number of characters. This work is mostly concerned with the determination of more than 80 characters for the 40 new serotypes identified since 1990. Several important characteristics were identified by this evaluation (Table 3).
Table 3. Biochemical characters of serovars identified since 1990
These are the results for the 46 serovars identified since 1990 and thus, not reported in a previous study (de Barjac & Frachon 1990) namely serotypes H28 to H67, plus seven subgroups.
Characters for which tests gave a positive reaction in all Bacillus thuringiensis serovars tested, with the exception of H51 which was negative for starch, glycogen and ribose, and H67 which was negative for ribose.
†Characters for which the tests gave a negative reaction with the exception of H65 which was galactose+.
Discriminant characters are those that may differ between serovars.
starch, gelatin, glycogen,
Acetyl-methyl-carbinol production (VP)
Tryptophan deaminase H2S production
Utilization of citrate
glucose, fructose, maltose,
galactose, lactose, mannitol
sucrose, mannose, arbutin, salicin,
The biochemical characters were roughly grouped into three major categories. The first consisted of characters positive for all serovars, as indicated in Table 3. The second grouped together those characters that are generally negative. This category includes the use of many sugars (35 of the 50 substrates of the API 50 CH galleries), and several enzymes, such as lysine decarboxylase (LDC), ornithine decarboxylase (ODC), tryptophan deaminase (TDA), β-galactosidase and indole production. Only one (H24a24b) of the 3500 B. thuringiensis isolates of the IEBC collection contained β-galactosidase and fermented galactose, whereas the other known strains of this serovar, and strains of serovar novosibirsk (H24a24c), did not. Indole production was not detected in any of the B. thuringiensis strains of the collection.
The third category grouped together characters which are taxonomically useful because they act as discriminant factors between serovars. The main factors in the group were: the presence of arginine dihydrolase (ADH) or enzymes for reduction of nitrates, urease, and the ability to ferment sucrose, mannose, cellobiose or salicin. Also included in this group is the production of acetyl-methyl-carbinol (AMC) that is negative only in a few cases. These are precisely the elements of the key previously described (de Barjac & Frachon 1990). Such characters were used to compare the various antigenic subgroups of the serotypes tested, and to compare several isolates for particular serovars.
Such discriminant characters (e.g. ADH, urease, sucrose and mannose) may differ between serovars of the same serotype, but with different sub-antigenic characterization. Isolates from a single serovar may also differ from each other in one or two characters. For example, in serovar morrisoni (H8a8b), six isolates were identical for the major characters AMC, CIT utilization, NO3 reduction, sucrose and mannose utilization, but differed in ADH production. Two of the six strains were ADH+, the others being ADH− (including the reference strain morrisoni), and these two strains have activity against Coleoptera. Only one of the six strains was positive for mannose. Many more samples must be compared before it will be possible to conclude that most of the discriminant characters are the same within a given serovar. However, the discriminant characters for the serovar kurstaki appear to be homogeneous.
Finally, from these investigations conducted mainly with newly characterized serovars, it is concluded that the biochemical key is still of value, but it cannot be used in isolation to differentiate between or within serovars. This procedure used in conjunction with H-serotyping does help to distinguish between several possibilities when serotyping is unclear.
There are also technical considerations concerning reliability of micromethods, particularly the API systems. Such methods are effective for many characters, particularly for sugar utilization, and the results are consistent with those obtained by classical methods. For a very small number of characters (two or three), there may be some ambiguity. This is the case for urease, AMC production and sometimes also for ADH. Differences in the substrates used for the two methods may account for conflicting results. It is therefore advisable to use classical methods as well as micromethods in a few cases.
Crystal morphology and δδ-endotoxin profiles
The morphology and biochemical features of bacterial species are among the classical phenotypic properties used to define taxa. This may be valuable for parasporal inclusions which are also responsible for the larvicidal activity of B. thuringiensis strains. The presence or absence of crystals is the major criterion for distinguishing between B. thuringiensis and B. cereus. Therefore, it is very important to be able to detect crystals and to determine their morphology, which, in some cases, may reflect a kind of specificity because that crystal may have various forms, depending on subunit composition. Sporulating cultures of the reference strains of serotypes H30 to H67, as well as of subgroups 10a10c, 18a18c, 24a24c and 28a28c, were examined by microscopy as described in Material and Methods. The protein profiles of crystal components were determined by SDS-PAGE analysis, useful for characterizing δ-endotoxin families; some of them are presented in Figs 1 and 2. As the number of strains and serovars increases, it should be possible to gain more insight into the evolution of such characters.
About 40% of the reference strains produced crystals with classical morphology, either bipyramidal or cuboid (Table 4). The proteins of these crystals are well characterized and are typical of strains active against lepidopteran species (although a precise target has not necessarily been determined). A much smaller percentage of strains had spherical crystals that were closely associated or stacked, like those of B. thuringiensis israelensis. This shape was correlated with a particular protein profile. Only one of the 42 reference strains had large flat rectangular crystals with a protein profile typical of the tenebrionis strain. However, a large proportion (more than 50%) of the reference strains produced atypical crystals, often heterogeneous in size and shape with protein profiles consisting of many poorly defined components, or novel profiles including polypeptides of 30–50 kDa in size, or larger than 160 kDa, as reported by several authors (Juarez-Perez et al. 1994; Burtseva et al. 1995; Chaufaux et al. 1997). The results of SDS-PAGE analysis referring to crystal proteins from the reference strains of 18 different serovars are shown in Figs 1 and 2. These strains are among those which display atypical and heterogeneous crystals. A great diversity of profiles was observed, some indicating components lower than 60 kDa (Fig. 1, lanes 4, 5, 6 and Fig. 2, lane 1), and colleagues showing multiple components (Fig. 1, lanes 8, 10 and Fig. 2, lane 7) or components ranging between 100 and 65 kDa (Fig. 2, lanes 4, 5, 6); components higher than 160 kDa, as seen in Fig. 2, lane 8 (indicating a polypeptide of about 200 kDa), appeared much less frequently. Most of these different crystal components have not yet been characterized. These figures also indicate that two subgroups of a same serotype may have crystals with very different profiles (Fig. 1, lanes 3 and 4).
In most cases, and typically for serotypes described in the last 10 years, no target insects have been identified. The mosquitocidal activity has been determined for the 40 strains. The jegathesan (H28a28c) (Seleena et al. 1995), medellin (H30) (Orduz et al. 1992) and one other strain had high levels of mosquitocidal activity (with the expected protein profiles), and four other strains presented a weak but significant activity against Culex or Aedes species (Ragni et al. 1996). In these four strains, the observed activity was correlated with the presence of components cross-reacting with Cry4B, Cry11 A or CytA (unpublished data). This also makes it impossible to determine the relationship between serovars and pathovars. However, there are several correlations involving previously characterized serotypes (before H28).
Several methods of investigation were used, including the detection of several δ-endotoxin gene-products, to obtain information about crystal components.
(i) It is clear from many studies, as reviewed by Delécluse et al. (1996), that most of the highly active mosquitocidal strains belong to H14 and all have the same subunit composition. Other very active strains were identified among new serotypes (after H28a28b) as described above. Strains with high or moderate activity were found among well characterized serotypes (H5a5c, H10a10b, H12) and they had various subunit structures (Ragni et al. 1996). Other strains with low activity were also detected (Ishii & Ohba 1993). Thus, mosquitocidal strains have been classified within four groups, from highly toxic to low toxicity (Ohba et al. 1995; Delécluse et al. 1996; Ragni et al. 1996).
(ii) It has been regularly observed that new strains with activity against Spodoptera littoralis and S. exigua belong mostly to two main serotypes, H6 (entomocidus) and H7 (aizawai), which contain the cry1C gene (unpublished). However, it cannot be inferred from this observation that all strains of these serovars have the same activity, or that such activity does not exist in other serotypes. A strain of serotype H1 containing the Cry1C δ-endotoxin and the corresponding gene has recently been identified (data not published). Strains with moderate activity were also detected among various serovars, kenyae (H4a4) or tolworthi (H9) for example, in which cry1E or variants were detected (data not published).
(iii) Another striking example frequently reported is serovar morrisoni (H8a8b) that brings together the highly mosquitocidal strain, PG14, which has crystal components similar to those of B. thuringiensis israelensis, other strains such as strain tenebrionis with activity against Coleoptera and typical subunit composition, and some strains active against Lepidoptera.
Thus, there are many types of relationships between the major known pathotypes and the H classification. However, there are some invariant features which must be kept in mind.
The B. thuringiensis classification and related problems
The H-serotyping method, as currently defined and used, provides a simple and efficient tool for classifying strains of B. thuringiensis species, the characteristic of which is the presence of one or more parasporal inclusions. However, there are at least two problematic situations: (i) strains lacking a parasporal inclusion and thus considered to be B. cereus and (ii) ‘autoagglutinated strains’. As the number of B. thuringiensis strains in collection steadily increases, the number of isolates falling into these two categories also increases in the same proportion.
Some B. cereus strains have antigens that cross-react with sera specific for B. thuringiensis H-serotypes (de Barjac & Bonnefoi 1973; Ohba & Aizawa 1986). Some of the B. cereus isolates of the IEBC Collection were serotyped (Table 5). Ninety-two of the 194 isolates were agglutinated by antisera specific for various B. thuringiensis serotypes, of which the most representative were H14, H5, H10, H6 and H27. Such isolates may originate from ancient B. thuringiensis strains that have lost plasmid-encoded crystals. They may also be true B. cereus with antigens in common with B. thuringiensis. This notion is credible, assuming that B. cereus and B. thuringiensis have common phenotypic characters and are almost identical in terms of phylogenetic characters, as for 16 s RNA sequences (Ash et al. 1991). Investigation of more characters, particularly plasmid patterns and additional biochemical characters, is required to determine which of the two alternatives applies.
Table 5. Identification of Bacillus thuringiensis (Bt) H-serotypes among B. cereus strains
Number of strains
Percentage of total
These are the results for strains identified as B. cereus after 1993 and numbered as CER 616 to CER 810 at the IEBC.
Percentage of total (92) cross-reacting isolates for each of the designated serovars.
Similar results were obtained with populations of B. cereus strains from natural sources not included in the IEBC collection (Helgason et al. unpublished); 55% of the isolates studied had antigens in common with B. thuringiensis serotypes.
Serotyping cannot always be performed in true B. thuringiensis strains with normal inclusion bodies. ‘Autoagglutinated’ strains are not typeable. These strains make up almost 3% of the B. thuringiensis in the IEBC collection.
Cultures of these strains agglutinated spontaneously, particularly when subjected to the conditions required for the test at all growth stages, thereby making serotyping impossible. The reasons for this are not clear, but this is the only situation in which the H-classification is completely useless. For different reasons, a few B. thuringiensis strains, called non-motile strains, also escape H-serotyping (de Barjac & Frachon 1990).
The main aim of this paper was to update the B. thuringiensis classification based on H-flagellar antigens, and to assess the current validity of the method. We also compared our methods with others currently in use, including molecular techniques, to try to determine the most useful methods for future studies.
There has been a large increase in the number of serovars consistent with the increasing number of B. thuringiensis strains of the IEBC collection. The WHO Collaborating Centre for Entomopathogenic Bacillus is responsible for making statements on this point. We have thus made this information official to avoid confusion in numbering and naming new serotypes of B. thuringiensis as previously suggested by Burges et al. (1982). This work also demonstrated that the method efficiently classified B. thuringiensis isolates in most situations.
The test tube agglutination method is recommended (Kahn tubes or Micronic® tubes), at least for the titration step of identification. Titration could also be performed using a micromethod (Laurent et al. 1996) assuming that suitable automatic equipment is available for reading the plaques.
The set of reference strains and corresponding antisera could be used only for B. thuringiensis species as defined in Bergey's Manual (Sneath 1986). This requires a meticulous examination for the presence of crystals. There are mutants with no crystals resulting from in vivo mutagenesis or from plasmid curing of known B. thuringiensis strains. It is clear that such isolates, the origin of which is well established, cannot be considered to be B. cereus (de Barjac & Bonnefoi 1973). The situation for natural B. cereus isolates of various origins is quite different. If the method is applied to B. cereus strains, some (Ð45%) showed a positive relationship to B. thuringiensis H-antigens, whereas the others did not react at all. This is consistent with previous reports (Krieg 1969; Burges 1984; Ohba & Aizawa 1986; Helgason et al. unpublished). Further investigation is required as there may be genetic exchanges due to plasmid transfer. However, B. cereus is a widely dispersed species including heterogeneous subgroups.It would therefore be sensible to classify B. cereus strains possessing B. thuringiensis H-antigens into a subgroup B. cereus/B. thuringiensis H+ commonly treated as a variety of B. cereus. More detailed knowledge of the H-antigen genes would be very useful.
‘Autoagglutinated’ strains have eluded H-classification. For unknown reasons, H-antigenic suspensions of these strains agglutinate spontaneously in the absence of specific antiserum under the conditions of the test procedure. They are motile strains because it is possible to prepare antigenic suspensions. It would be of interest to determine the factors involved in such a process. This is one of the limitations of the H-classification. The biochemical and genetic basis of the flagellar antigens system in B. thuringiensis is totally unknown and should be treated as a priority for investigation.
This work also stressed the difficulties in predicting any correlation between serotypes and pathotypes. However, it was still possible to establish a rough correlation between a pathotype and several well known serotypes or serovars. The diversity and the extreme variability of the δ-endotoxin genes (more than 100 genes have been cloned and more than 22 δ-endotoxin families and subgroups defined by Crickmore et al. 1998), and the frequent presence of multiple toxin genes in individual isolates, results in an infinite number of potential gene combinations, making any B. thuringiensis classification based on pathotypes impossible. In addition, very few serotypes identified within the last 10 years have a known, specific, target insect.
The value of biochemical characters was also examined in detail. A key was previously established (de Barjac & Frachon 1990) to differentiate groups of strains on the basis of determinant characters, with the aid of computer analysis involving about 80 characters. This rapidly proved to be inefficient for distinguishing between serovars. As the number of strains tested increases, it appears that some of the rare discriminant characters may vary not only between serovars, but also among strains of the same serovar. However, when conflicting serovar identification results are obtained, biochemical characters may provide additional discriminating information. Thus, there are situations in which biochemical characters may be of value if used in conjunction with other methods.
Other phenotypic methods also have varying levels of success. For example, cellular fatty acid patterns are known to have high taxonomic resolution at the species level. This method, a chemotaxonomic method (Buss et al. 1996), with a high degree of automation, successfully differentiates groups within the B. sphaericus species (Frachon et al. 1991), whereas it cannot distinguish between B. thuringiensis subspecies or between B. thuringiensis and B. cereus (Frachon and Lecadet unpublished). Another approach based on susceptibility to different bacteriophages was developed by Ackerman et al. (1995) and resulted in the identification of phage types. As indicated by the authors, there was no correlation between H-antigen serotype and the 25 phagovars determined at that time, which showed frequent cross-reactions with B. cereus.
Many genotypic methods, widely used as tools for molecular taxonomy, suggest that there is a very close relationship between B. thuringiensis, B. cereus and even B. anthracis. Some distinguish, with various levels of success, B. cereus and B. thuringiensis. Giffel et al. (1997) reported possible discrimination between the two species, using specific DNA probes based on particular variable regions of 16 s RNA. Other studies (Bourque et al. 1995) suggest that the intergenic spacer region (ISA) is a discriminant probe.
Effective methods of subspecies analysis are needed to provide tools for a molecular biology-based classification. Several attempts using RFLP, DNA colony hybridization, RAPD or ribotyping, as reviewed by Damgaard (1996), have been reported. With regard to ribotyping, there are essentially two valuable approaches for investigating relationships between serotyping and this new method. Priest et al. (1994) examined 43 B. thuringiensis strains among 10 well known serovars and found a relatively good correspondence between some serovars and ribotype patterns, whereas a significant diversity of these patterns was observed. Another investigation reported by Akhurst et al. (1997) detected characteristic profiles for strains belonging to several widespread serovars (THU, KUR, ENT, AZA, MOR, TOL, DAR, TOU, ISR); in addition, their results indicated limited variations among strains within serovars that could be used as a tool to distinguish between strains, particularly between reisolates. In this line, the results of a preliminary investigation to cover the whole classification showed a diversification of ribotype patterns. Nevertheless, these appeared to be relatively constant within the serovars examined (THU, KUR, ISR) (Frachon and Delécluse, unpublished data). However, analysis of many more serotypes and strains within serovars is necessary. It is entirely possible that variability will increase with the number of strains examined. With regard to other molecular techniques, some, including DNA-colony hybridization and random amplified polymorphic DNA (RAPD) analysis, were investigated by Hansen et al. (1998). The two methods, which addressed different objectives, were found to be very informative, particularly with respect to screening procedures. Depending on the primers used, RAPD analysis could allow particular isolates to be distinguished within serovars, although profiles appear relatively characteristic of the serovars examined (mainly KUR). Once again, any comparison between these approaches and the phenotypic methods would require the study of many more strains within different serotypes. However, DNA colony hybridization, like RAPD, must be useful for grouping strains, either independently or in conjunction with others. There is a large scope for investigation in this field and much work is required for conclusions to be drawn.
Thus, the serotyping method based on H-antigens is still providing a valuable and reliable tool for discriminating between groups of B. thuringiensis strains. Consistent with previous work (Burges 1984; de Barjac & Frachon 1990), we found that serovars cannot be seen as subspecies, but rather as varieties constituting ‘clear subdivisional structures’ within the species, independently of crystal type. In the future, H-classification will have to be used in parallel with other methods, particularly for B. cereus cross-reacting strains and for ‘autoagglutinated’ strains for which serotyping is impossible. Developing simple molecular approaches should be of great value in these cases.
The need for accurate identification methods to differentiate between the continuously increasing number of B. thuringiensis strains requires the use of various complementary approaches, including molecular methods.
The authors are grateful to J.-F. Charles for critical reading of the manuscript, and to Alex Edelman for revising the English manuscript. Thanks are also given to Mrs M.-F. Blanc for typing and making up the manuscript. This work was supported by research funds from the Institut Pasteur.