You have full text access to this OnlineOpen article

Meso-scale ecology of anthrax in southern Africa: a pilot study of diversity and clustering

  1. K. L. Smith1,
  2. V. De Vos2,
  3. H. B. Bryden2,
  4. M. E. Hugh-Jones3,
  5. A. Klevytska1,
  6. L. B. Price1,
  7. P. Keim1,
  8. D. T. Scholl3

Article first published online: 25 DEC 2001

DOI: 10.1046/j.1365-2672.1999.00871.x

Issue

Journal of Applied Microbiology

Journal of Applied Microbiology

Volume 87, Issue 2, pages 204–207, August 1999

Additional Information

How to Cite

Smith, K. L., De Vos, V., Bryden, H. B., Hugh-Jones, M. E., Klevytska, A., Price, L. B., Keim, P. and Scholl, D. T. (1999), Meso-scale ecology of anthrax in southern Africa: a pilot study of diversity and clustering. Journal of Applied Microbiology, 87: 204–207. doi: 10.1046/j.1365-2672.1999.00871.x

Author Information

  1. 1

    Department of Biology, Northern Arizona University, Flagstaff, AZ, USA,

  2. 2

    Scientific Services, Skukuza, Kruger National Park, South Africa and

  3. 3

    Department of Epidemiology and Community Health, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA

* K.L. Smith, Department of Biology, Northern Arizona University, Flagstaff, AZ, USA.

Publication History

  1. Issue published online: 25 DEC 2001
  2. Article first published online: 25 DEC 2001
  3. Received and revised 15 May 1999 and accepted 29 May 1999

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (114K)

It has only recently been possible to detect sufficient genetic diversity among anthrax isolates to allow genotype grouping ( Keim et al. 1997 ). Early results of such grouping suggest that the southern African subcontinent may be the geographical origin of Bacillus anthracis. This report describes a pilot investigation of the genetic diversity of a study group of isolates from the Kruger National Park, South Africa, and efforts to detect spatio-temporal clustering within the study group. This study has also served as further validation for the newly developed Multi-Locus VNTR Analysis (MLVA), designed to simplify genotyping of B. anthracis isolates. The results reveal a diverse range of genotypes within the park allied with three genotype reference groups, and show that the MLVA procedure is robust for rapid analysis of B. anthracis genotypes. We also observed multiple genotype groups within epidemics and between geographically and temporally close epidemic episodes. This is in contrast to earlier characterizations of anthrax epidemics. The result of a Mantel test for time–space clustering indicates clustering of the anthrax isolates selected for the study.

View Full Article (HTML) Get PDF (114K)