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Antibody-based systems for the detection of Bacillus anthracis in environmental samples

  1. G. W. Long,
  2. T. O’Brien

Article first published online: 25 DEC 2001

DOI: 10.1046/j.1365-2672.1999.00877.x

Issue

Journal of Applied Microbiology

Journal of Applied Microbiology

Volume 87, Issue 2, page 214, August 1999

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How to Cite

Long, G. W. and O’Brien, T. (1999), Antibody-based systems for the detection of Bacillus anthracis in environmental samples. Journal of Applied Microbiology, 87: 214. doi: 10.1046/j.1365-2672.1999.00877.x

Author Information

  1. Biological Defence Research Program, Naval Medical Research Institute, Bethesda, MD, USA

* G.W. Long, Biological Defence Research Program, Naval Medical Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20814, USA.

Publication History

  1. Issue published online: 25 DEC 2001
  2. Article first published online: 25 DEC 2001
  3. Received and revised 15 May 1999 and accepted 29 May 1999

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There is a necessity for rapid immunodiagnostic techniques for the detection and identification of Bacillus anthracis in environmental specimens. The technology available for accomplishing this ranges in complexity from a simple dipstick type assay to complex biosensors. We have developed antigen capture dipstick assays for a series of infectious agents including an assay for B. anthracis protective antigen and one for B. anthracis spores. These immunochromatographic assays use colloidal gold to visualize the reaction and take approximately 15 min to perform. We will also describe our current effort in the development of two antigen detection biosensors and discuss the sensitivity and specificity of the assays in environmental specimens.

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