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Molecular recognition specificity of Bacillus anthracis spore antibodies

  1. P. Longchamp,
  2. T. Leighton

Article first published online: 25 DEC 2001

DOI: 10.1046/j.1365-2672.1999.00879.x

Issue

Journal of Applied Microbiology

Journal of Applied Microbiology

Volume 87, Issue 2, pages 246–249, August 1999

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How to Cite

Longchamp, P. and Leighton, T. (1999), Molecular recognition specificity of Bacillus anthracis spore antibodies. Journal of Applied Microbiology, 87: 246–249. doi: 10.1046/j.1365-2672.1999.00879.x

Author Information

  1. Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA

* T. Leighton, Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, CA 94720-3203, USA (e-mail: Leighton@Bacillus.Berkeley.Edu).

Publication History

  1. Issue published online: 25 DEC 2001
  2. Article first published online: 25 DEC 2001
  3. Received and revised 15 May 1999 and accepted 29 May 1999

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The sensitivity and specificity of polyclonal and monoclonal antibodies raised against anthrax spore preparations has been assessed by Western blotting. None of the antibodies studied were completely specific in recognizing the anthrax spore surface. A polyclonal serum recognized a wide range of spore surface epitopes and demonstrated limited cross-reaction with the near-neighbour species Bacillus cereus spore surface. Two monoclonal antibodies studied demonstrated more extensive cross-reaction with distant-neighbour species B. globigii and B. subtilis. These monoclonal antibodies did not react with spore surface epitopes but did bind strongly to vegetative cell epitopes in all four Bacillus species studied.

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