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Detection and characterization of a bacteriocin, garviecin L1-5, produced by Lactococcus garvieae isolated from raw cow’s milk
Article first published online: 15 MAR 2002
DOI: 10.1046/j.1365-2672.2001.01261.x
1365-2672/asset/cover.gif?v=1&s=c77114a65c2d4b5cc5501f9ca1a6c03e826dec22 "Journal of Applied Microbiology")
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How to Cite
Villani, F. , Aponte, M. , Blaiotta, G. , Mauriello, G. , Pepe, O. and Moschetti, G. (2001), Detection and characterization of a bacteriocin, garviecin L1-5, produced by Lactococcus garvieae isolated from raw cow’s milk. Journal of Applied Microbiology, 90: 430–439. doi: 10.1046/j.1365-2672.2001.01261.x
Publication History
- Issue published online: 15 MAR 2002
- Article first published online: 15 MAR 2002
- Abstract
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Aims: The identification of a bacteriocin-producing lactococcal strain isolated from raw cow’s milk is reported, along with production conditions, physical and chemical properties, and mode of action of the bacteriocin.
Methods and Results: On the basis of resistance to clindamycin, species-specific PCR and amplification of the 16S-23S rDNA spacer region, the strain was identified as Lactococcus garvieae. Its bacteriocin, designated garviecin L1-5, was bactericidal against closely related species and strains of species from different genera, including Listeria monocytogenes and Clostridium spp. Garviecin L1-5 was shown to be proteinaceous by protease inactivation and was unaffected by heat treatments, also at low pH values. When amplifying known lactococcal bacteriocin genes using DNA from strain L1-5 as template, no amplification products were observed on the agarose gel. The molecular weight of garviecin L1-5 was about 2·5 kDa. As far as is known, no bacteriocins have been detected from Lactococcus garvieae.
Conclusions: The general properties of garviecin L1-5 are characteristic of the low-molecular-weight bactericidal peptide group.
Significance and Impact of the Study: The survey of micro-organisms for novel antimicrobial substances provided valuable information on their physiology, ecology and practical application.