Comparative study of nine Lactobacillus fermentum bacteriophages
Article first published online: 21 DEC 2001
Journal of Applied Microbiology
Volume 91, Issue 3, pages 394–403, September 2001
How to Cite
Foschino, R. , Picozzi, C. and Galli, A. (2001), Comparative study of nine Lactobacillus fermentum bacteriophages. Journal of Applied Microbiology, 91: 394–403. doi: 10.1046/j.1365-2672.2001.01392.x
- Issue published online: 21 DEC 2001
- Article first published online: 21 DEC 2001
Aims: To investigate the basic properties of six temperate and three virulent phages, active on Lactobacillus fermentum, on the basis of morphology, host ranges, protein composition and genome characterization.
Methods and Results: All phages belonged to the Siphoviridae family; two of them showed prolate heads. The host ranges of seven phages contained a common group of strains. SDS-PAGE protein profiles, restriction analysis of DNA and Southern blot hybridization revealed a high degree of homology between four temperate phages; partial homologies were also detected among virulent and temperate phages. Clustering derived from host range analysis was not related to the results of the DNA hybridizations.
Conclusions: The phages investigated have common characteristics with other known phages active on the genus Lactobacillus. Sensitivity to viral infection is apparently enhanced by the presence of a resident prophage.
Significance and Impact of the Study: These relationships contribute to the explanation for the origin of phage infection in food processes where Lact. fermentum is involved, such as sourdough fermentation.