The use of LenticulesTM for the process control of enumeration techniques in food and environmental microbiology
Article first published online: 7 JUL 2008
Journal of Applied Microbiology
Volume 91, Issue 4, pages 660–667, October 2001
How to Cite
Lightfoot, N.F. , Richardson, I.R. and Harford, J.P. (2001), The use of LenticulesTM for the process control of enumeration techniques in food and environmental microbiology. Journal of Applied Microbiology, 91: 660–667. doi: 10.1046/j.1365-2672.2001.01432.x
- Issue published online: 7 JUL 2008
- Article first published online: 7 JUL 2008
Aims: Lenticules™consit of control-dried plano convex discs in which biologically-active materials are contained within a water-soluble matrix. They can be produced to contain stable numbers of bacteria from 10 cfu lenticule–1 to 108 cfu lenticule–1 with a wide variety of organisms. These experiments were carried out to validate their use as a tool for internal quality control in quantitative microbiology.
Methods and Results: The Lenticules™ were used routinely in standard quantitative microbiological procedures across five laboratories. Results showed the materials to be stable, homogenous and capable of identifying systematic errors.
Conclusions: The Lenticules™ provide suitable, stable control materials.
Significance and Impact of the Study: Routine internal quality control of quantitative measurements is greatly improved; the materials are easy to use and enable comparisons between laboratories to be made.