Aims: The study aimed to investigate the survival characteristics of Escherichia coli O157:H7 in farm water (FW), and in sterile distilled municipal water (SDW), stored outdoors under field conditions, with or without the addition of faeces (1% w/v), in a farmyard shed and the laboratory at 15°C. Methods and Results: Water samples were inoculated with E. coli O157:H7 at 103 and 106 ml−1, and sampled over a 31-day period. In FW stored outdoors in a field, E. coli O157:H7 survived for 14 days at temperatures <15°C, at both inoculation levels, while in the laboratory at 15°C, the organism was still detectable at low levels (<1 log10 cfu ml−1) after 31 days. The addition of bovine faeces to water outdoors (1% w/v) resulted in survival for 24 days. In SDW inoculated at 106 ml−1 and stored in the laboratory (15°C), only a 2·5 log reduction was observed after 31 days, while the organism could not be detected after 17 days in the field. Preliminary screening of water samples stored outdoors isolated a bacterium which exhibited antimicrobial activity towards E. coli O157:H7. Conclusions: The survival of E. coli O157:H7 observed in this study illustrates the potential of farm water to act as a vehicle in the transfer of the organism across a herd. Significance and Impact of the Study: The difficulty in extrapolating results from controlled laboratory situations to on-farm conditions is also highlighted in this study.