The sensitivity to honey of Gram-positive cocci of clinical significance isolated from wounds
Article first published online: 23 OCT 2002
Journal of Applied Microbiology
Volume 93, Issue 5, pages 857–863, November 2002
How to Cite
Cooper, R.A., Molan, P.C. and Harding, K.G. (2002), The sensitivity to honey of Gram-positive cocci of clinical significance isolated from wounds. Journal of Applied Microbiology, 93: 857–863. doi: 10.1046/j.1365-2672.2002.01761.x
- Issue published online: 23 OCT 2002
- Article first published online: 23 OCT 2002
- 2002/38: received 21 January 2002, revised 26 July 2002 and accepted 16 August 2002
Aims: To determine the sensitivity to honey of Gram-positive cocci of clinical significance in wounds and demonstrate that inhibition is not exclusively due to osmotic effects.
Methods and Results: Eighteen strains of methicillin-resistant Staphylococcus aureus and seven strains of vancomycin-sensitive enterococci were isolated from infected wounds and 20 strains of vancomycin-resistant enterococci were isolated from hospital environmental surfaces. Using an agar incorporation technique to determine the minimum inhibitory concentration (MIC), their sensitivity to two natural honeys of median levels of antibacterial activity was established and compared with an artificial honey solution. For all of the strains tested, the MIC values against manuka and pasture honey were below 10% (v/v), but concentrations of artificial honey at least three times higher were required to achieve equivalent inhibition in vitro. Comparison of the MIC values of antibiotic-sensitive strains with their respective antibiotic-resistant strains demonstrated no marked differences in their susceptibilities to honey.
Conclusions: The inhibition of bacteria by honey is not exclusively due to osmolarity. For the Gram-positive cocci tested, antibiotic-sensitive and -resistant strains showed similar sensitivity to honey.
Significance and Impact of the Study: A possible role for honey in the treatment of wounds colonized by antibiotic-resistant bacteria is indicated.