The complete nucleotide sequence of the Vibrio harveyi bacteriophage VHML
Article first published online: 23 NOV 2002
Journal of Applied Microbiology
Volume 93, Issue 6, pages 1089–1098, December 2002
How to Cite
Oakey, H.J., Cullen, B.R. and Owens, L. (2002), The complete nucleotide sequence of the Vibrio harveyi bacteriophage VHML. Journal of Applied Microbiology, 93: 1089–1098. doi: 10.1046/j.1365-2672.2002.01776.x
- Issue published online: 23 NOV 2002
- Article first published online: 23 NOV 2002
- 2002/322: received 5 September 2002, revised 16 September 2002 and accepted 30 September 2002
Aims: To determine the complete nucleotide sequence of the bacteriophage VHML and establish a hypothesis for the virulence conversion caused by VHML infection of Vibrio harveyi.
Methods and Results: The complete nucleotide sequence of VHML was determined (43 193 bp) and used to identify putative genes. The translated products of these genes were compared with reported sequences to assign hypothetical functions. All anticipated structural genes and putative genes for lysogeny were identified. In addition, we found a complete N6-adenine methyltransferase (Dam) gene that appeared to have an essential site for ADP-ribosylating toxins at the C-terminal of the translated product.
Conclusions: Virulence conversion of V. harveyi by VHML may be associated with Dam transcriptional regulation. The Dam gene may also encode for a toxin component similar to ADP-ribosylating toxins.
Significance and Impact of Study: This manuscript lays the foundation for understanding the virulence of toxin-producing V. harveyi. Further research into aspects discussed here will lead to a greater comprehension regarding the invertebrate disease vibriosis and its control in the farming of these animals.