Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark
Article first published online: 10 APR 2003
Journal of Applied Microbiology
Volume 94, Issue 5, pages 929–935, May 2003
How to Cite
Lund, M., Wedderkopp, A., Wainø, M., Nordentoft, S., Bang, D.D., Pedersen, K. and Madsen, M. (2003), Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark. Journal of Applied Microbiology, 94: 929–935. doi: 10.1046/j.1365-2672.2003.01934.x
- Issue published online: 10 APR 2003
- Article first published online: 10 APR 2003
- 2002/395: received 16 October 2002, revised 11 February 2003 and accepted 11 February 2003
- DNA isolation;
Aims: To develop and evaluate a rapid and sensitive PCR method for detection of Campylobacter spp. directly from chicken faeces.
Methods and Results: DNA was isolated from faecal swabs using magnetic beads followed by PCR using a prealiquoted PCR mixture, which had been stored in the freezer. The result could be obtained in <6 h. The method was evaluated on 1282 samples from the Danish surveillance programme for Campylobacter in broilers by comparing with conventional culture. The diagnostic specificity was calculated to be 0·99. The detection limits of the PCR method and of the conventional culture were compared using spiked control material. For both methods the detection limit was 36 CFU ml−1.
Conclusions: It was concluded that the PCR proved useful for detection of Campylobacter in pooled cloacal swabs from broilers.
Significance and Impact of the Study: By taking cloacal samples in the broiler flocks the technique can be used as an important tool for planning and directing the broiler slaughtering process. This will be a great help in minimizing the risk of contaminating Campylobacter-free flocks at the abattoir.