You have full text access to this OnlineOpen article

The effect of bovine lactoferrin and lactoferricin B on the ability of feline calicivirus (a norovirus surrogate) and poliovirus to infect cell cultures

  1. K.B. McCann1,
  2. A. Lee2,
  3. J. Wan2,
  4. H. Roginski2,
  5. M.J. Coventry2

Article first published online: 13 OCT 2003

DOI: 10.1046/j.1365-2672.2003.02071.x

Issue

Journal of Applied Microbiology

Journal of Applied Microbiology

Volume 95, Issue 5, pages 1026–1033, November 2003

Additional Information

How to Cite

McCann, K., Lee, A., Wan, J., Roginski, H. and Coventry, M. (2003), The effect of bovine lactoferrin and lactoferricin B on the ability of feline calicivirus (a norovirus surrogate) and poliovirus to infect cell cultures. Journal of Applied Microbiology, 95: 1026–1033. doi: 10.1046/j.1365-2672.2003.02071.x

Author Information

  1. 1

    Institute of Land and Food Resources, The University of Melbourne, Werribee, Victoria

  2. 2

    Food Science Australia, Werribee, Victoria, Australia

*M.J. Coventry, Food Science Australia, Private Bag 16, Sneydes Road, Werribee, Victoria 3030, Australia (e-mail: john.coventry@foodscience.afisc.csiro.au).

Publication History

  1. Issue published online: 13 OCT 2003
  2. Article first published online: 13 OCT 2003
  3. 2002/437: received 8 November 2002, revised and accepted 3 July 2003

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (133K)

Keywords:

  • feline calicivirus;
  • lactoferricin B;
  • lactoferrin;
  • poliovirus

Abstract

Aims: To characterize the effect of bovine lactoferrin and lactoferricin B against feline calicivirus (FCV), a norovirus surrogate and poliovirus (PV), as models for enteric viruses.

Methods and Results: Crandell-Reese feline kidney (CRFK) cells were used for the propagation of FCV and monkey embryo kidney (MEK) cells for PV. The assays included visual assessment of cell lines for cytopathic effects and determination of the percentage cell death using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium] dye reduction assay. Incubation of bovine lactoferrin with CRFK cells either prior to or together with FCV inoculation substantially reduced FCV infection. In contrast, the interference of lactoferrin with the infection of cells with PV was demonstrated only when lactoferrin was present with cell lines and virus for the entire assay period. Using indirect immunofluorescence, lactoferrin was detected on the surface of both CRFK and MEK cells, suggesting that the interference of viral infection may be attributed to lactoferrin binding to the surfaces of susceptible cells, thereby preventing the attachment of the virus particles. Lactoferricin B, a cationic antimicrobial peptide derived from the N-terminal domain of bovine lactoferrin, reduced FCV but not PV infection.

Conclusion: Lactoferrin was shown to interfere with the infection of cells for both FCV and PV. However, lactoferricin B showed no interference of infection with PV and interference with infection for FCV required the presence of lactoferricin B together with the cell line and virus.

Significance and Impact of the Study: An in vitro basis is provided for the effects of bovine lactoferrin and lactoferricin B in moderating food-borne infections of enteric viruses.

View Full Article (HTML) Get PDF (133K)