Action of trehalose on the preservation of Lactobacillus delbrueckii ssp. bulgaricus by heat and osmotic dehydration
Article first published online: 22 OCT 2003
Journal of Applied Microbiology
Volume 95, Issue 6, pages 1315–1320, December 2003
How to Cite
Gómez Zavaglia, A., Tymczyszyn, E., De Antoni, G. and Aníbal Disalvo, E. (2003), Action of trehalose on the preservation of Lactobacillus delbrueckii ssp. bulgaricus by heat and osmotic dehydration. Journal of Applied Microbiology, 95: 1315–1320. doi: 10.1046/j.1365-2672.2003.02102.x
- Issue published online: 22 OCT 2003
- Article first published online: 22 OCT 2003
- 2003/332: received 21 April 2003, revised and accepted 15 August 2003
- air drying;
- lactic bacteria;
- osmotic response;
- water permeability;
- zeta potential
Aim: This work determines the efficiency of trehalose on the preservation by heat or osmotic drying of a strain of Lactobacillus delbrueckii ssp. bulgaricus. Cell recovery at different trehalose concentrations during drying correlated with the surface properties and osmotic response of cells after rehydration.
Methods and Results: Bacteria were dried in the presence of glycerol, trehalose, sucrose at 70°C and at 20°C. Trehalose attenuates the loss of viability at 0·25 m. At this concentration, the osmotic response and zeta potential of the bacteria were comparable with the nondried ones.
Conclusions: Trehalose diminishes significantly the damage produced by dehydration both when the bacteria are dried by heating or subjected to osmotic dehydration. This effect appears related to the preservation of the permeability to water and the surface potential of the bacteria.
Significance and Impact of the Study: Dehydration occurring during heating or during osmosis appears to have similar effects. As dehydration-induced damage is in correlation with osmotic response recovery and is hindered or buffered by the presence of trehalose, it may be related to water eliminated from biological structures involved in water permeation.