Evaluating the performance of fluorescence microscopes
Article first published online: 5 JAN 2002
Journal of Microscopy
Volume 191, Issue 2, pages 128–134, August 1998
How to Cite
Murray (1998), Evaluating the performance of fluorescence microscopes. Journal of Microscopy, 191: 128–134. doi: 10.1046/j.1365-2818.1998.00374.x
- Issue published online: 5 JAN 2002
- Article first published online: 5 JAN 2002
- Fluorescence microscopy;
- light throughput;
- performance evaluation;
- signal-to-noise ratio.
A simple means of evaluating the performance of fluorescence microscopes is described. The proposed test gives an overall figure of merit that takes into account all of the important instrumental parameters that determine image quality. The essence of the test is to use a specimen whose photobleaching rate is a measure of the illumination time–intensity integral. When this time–intensity integral is controlled, the signal-to-noise ratio in the image of small subresolution objects becomes a system-independent measure of light throughput, resolution and intrinsic noise.