Wide-field optically sectioning fluorescence microscopy with laser illumination

Authors


Professor Tony Wilson Fax: + 44 (0)1865 273905; e-mail: tony.wilson@eng.ox.ac.uk

Abstract

We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one-dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an image of the grid pattern to be projected into the specimen. Images taken at three spatial positions of the grid are processed in a simple manner to provide optically sectioned images of fluorescent specimens.

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