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Keywords:

  • Anopheles gambiae complex;
  • malaria vectors;
  • molecular markers;
  • mosquito identification;
  • PCR-RFLP;
  • sibling species;
  • Africa

Abstract For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.