The work described in this report is part of the research developed by C. Palacios to fulfil the requirements to obtain a PhD in Molecular and Evolutionary Genetics at the University of Valencia, under the supervision of Dr González-Candelas. The main interest of this group is the application of population genetics and evolutionary biology to the conservation of endangered plant species through the use of different molecular markers. We currently work on several endemic species from the genus Limonium. Part of this work is performed in close collaboration with the Plant Biology Department and the Botanical Garden from the University of Valencia. Dr Kresovich's research has focused on the application of genomics to resolve questions of agricultural resources conservation and use. He currently serves as Director of the Institute for Genomic Diversity at Cornell University.
A population genetic study of the endangered plant species Limonium dufourii (Plumbaginaceae) based on amplified fragment length polymorphism (AFLP)
Article first published online: 28 JUN 2008
1999 Blackwell Science Ltd
Volume 8, Issue 4, pages 645–657, April 1999
How to Cite
Palacios, C., Kresovich, S. and González-Candelas, F. (1999), A population genetic study of the endangered plant species Limonium dufourii (Plumbaginaceae) based on amplified fragment length polymorphism (AFLP). Molecular Ecology, 8: 645–657. doi: 10.1046/j.1365-294X.1999.t01-1-00597.x
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- conservation genetics;
- endangered species;
- genetic variation;
- population structure
Limonium dufourii (Plumbaginaceae) is a triploid species with obligate apomictic reproduction and is endemic to the East Mediterranean coast of Spain, where it is present in only six populations, most of which have a very low number of individuals. Genetic variation and population structure in this species was studied using amplified fragment length polymorphisms (AFLPs) as markers, using the same individuals as in a previous study with random amplified polymorphic DNA (RAPD). Three different primers provided 252 bands of which 51 were polymorphic among the 152 individuals analysed. Those polymorphic bands were able to define 65 different phenotypes, of which all but two were present in only one population. The comparative analyses of data from AFLPs with those from RAPDs show a high degree of concordance. Additionally, and given the nature of these markers, we propose the estimation of nucleotide divergences from AFLP patterns. Relationships among the different AFLP patterns and the estimates of population genetic parameters obtained with this evolutionary distance are in good agreement with previous results. These analyses show that substantial genetic variability and differentiation exist within and among populations of L. dufourii. Their higher reproducibility and the possibility of obtaining estimates of nucleotide divergence make AFLPs a much better DNA fingerprinting technique.