M. M. Ribeiro and S. Mariette contributed equally to this work.
Comparison of genetic diversity estimates within and among populations of maritime pine using chloroplast simple-sequence repeat and amplified fragment length polymorphism data
Article first published online: 29 APR 2002
Volume 11, Issue 5, pages 869–877, May 2002
How to Cite
Ribeiro, M. M., Mariette, S., Vendramin, G. G., Szmidt, A. E., Plomion, C. and Kremer, A. (2002), Comparison of genetic diversity estimates within and among populations of maritime pine using chloroplast simple-sequence repeat and amplified fragment length polymorphism data. Molecular Ecology, 11: 869–877. doi: 10.1046/j.1365-294X.2002.01490.x
This study is part of MM Ribeiro’s PhD thesis about the genetic structure of Pinus pinaster by using nuclear and cytoplasmic markers. S Mariette compared different molecular markers to assess genetic diversity of different tree species. AE Szmidt is working with the application of molecular approaches to the study of plant evolutionary biology. GG Vendramin is involved in microsatellite marker analysis of many forest tree species. C Plomion is interested in the application of QTL analysis and mapping to operational tree breeding programme. A Kremer’s research is focused on the organization and dynamics of gene diversity in forest trees.
- Issue published online: 29 APR 2002
- Article first published online: 29 APR 2002
- Received 23 July 2001; revision received 21 January 2002; accepted 24 January 2002
- chloroplast microsatellites;
- gene diversity;
- population differentiation;
- Pinus pinaster
We compared the genetic variation of Pinus pinaster populations using amplified fragment length polymorphism (AFLP) and chloroplast simple-sequence repeat (cpSSR) loci. Populations’ levels of diversity within groups were found to be similar with AFLPs, but not with cpSSRs. The high interlocus variance associated with the AFLP loci could account for the lack of differences in the former. Although AFLPs revealed much lower genetic diversity than cpSSRs, the levels of among-population differentiation found with the two types of marker were similar, provided that loci showing fewer than four null-homozygotes, in any population, were pruned from the AFLP data. Moreover, the French and Portuguese populations were clearly differentiated from each other, with both markers. The Mantel test showed that the genetic distance matrix calculated using the AFLP data was correlated with the matrix derived from the cpSSRs. Because of the concordance found between markers we conclude that gene flow was indeed the predominant force shaping nuclear and chloroplastic genetic variation of the populations within regions, at the geographical scale studied.