Genetic differences among three colour morphotypes of the black rockfish, Sebastes inermis, inferred from mtDNA and AFLP analyses

  1. Yoshiaki Kai1,*,
  2. Kouji Nakayama1,
  3. Tetsuji Nakabo2

Article first published online: 9 OCT 2008

DOI: 10.1046/j.1365-294X.2002.01628.x

Issue

Molecular Ecology

Molecular Ecology

Volume 11, Issue 12, pages 2591–2598, December 2002

Additional Information

How to Cite

Kai, Y., Nakayama, K. and Nakabo, T. (2002), Genetic differences among three colour morphotypes of the black rockfish, Sebastes inermis, inferred from mtDNA and AFLP analyses. Molecular Ecology, 11: 2591–2598. doi: 10.1046/j.1365-294X.2002.01628.x

Author Information

  1. 1

    Graduate School of Agriculture, Division of Applied Biosciences, Kyoto University, Kitashirakawa-oiwake cho, Sakyo, Kyoto 606–8502, Japan,

  2. 2

    The Kyoto University Museum, Kyoto University, Kyoto 606–8501, Japan

* Y. Kai. Tel.: + 81-75-753-7731; Fax: + 81-75-753-3276; E-mail: kai@inet.museum.kyoto-u.ac.jp

Publication History

  1. Issue published online: 9 OCT 2008
  2. Article first published online: 9 OCT 2008
  3. Received 30 May 2002; revision received 7 August 2002; accepted 7 August 2002

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Keywords:

  • AFLP;
  • colour morphotypes;
  • hybridization;
  • incomplete lineage sorting;
  • mtDNA control region;
  • Sebastes inermis;
  • reproductive isolation

Abstract

The genetic differences among three colour morphotypes of the black rockish, Sebastes inermis, were determined from mitochondrial DNA (mtDNA) and amplified fragment length polymorphisms (AFLP) analyses. In the AFLP analysis, each morphotype could be distinguished by the presence or absence matrix of five AFLP loci. These diagnostic loci indicated that the three morphotypes represented independent gene pools, indicating reproductive isolation. Furthermore, 14 significant frequency differences in AFLP fragments were observed between morphotypes A and B, 12 between morphotypes A and C and six between morphotypes B and C. These significant differences also supported the likelihood of reproductive isolation among the morphotypes. In the mtDNA analysis, variations in partial sequences of the control region failed to distinguish clearly between the three morphotypes, but restrictions of gene flow and genetic differentiation among the morphotypes were supported by significant FST estimates. The absence of diagnostic mtDNA differences in this study may have been due to introgressive hybridization among the morphotypes and/or incomplete lineage sorting, due to the recency of speciation.

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