How much effort is required to isolate nuclear microsatellites from plants?
Article first published online: 25 APR 2003
Volume 12, Issue 6, pages 1339–1348, June 2003
How to Cite
Squirrell, J., Hollingsworth, P. M., Woodhead, M., Russell, J., Lowe, A. J., Gibby, M. and Powell, W. (2003), How much effort is required to isolate nuclear microsatellites from plants?. Molecular Ecology, 12: 1339–1348. doi: 10.1046/j.1365-294X.2003.01825.x
- Issue published online: 25 APR 2003
- Article first published online: 25 APR 2003
- Received 18 November 2002; revision received 23 January 2003; accepted 24 January 2003
- enriched library;
- molecular markers;
The attributes of codominance, reproducibility and high resolution have all contributed towards the current popularity of nuclear microsatellites as genetic markers in molecular ecological studies. One of their major drawbacks, however, is the development phase required to obtain working primers for a given study species. To facilitate project planning, we have reviewed the literature to quantify the workload involved in isolating nuclear microsatellites from plants. We highlight the attrition of loci at each stage in the process, and the average effort required to obtain 10 working microsatellite primer pairs.