A 5.8S nuclear ribosomal RNA gene sequence database: applications to ecology and evolution

  1. KENNETH W. CULLINGS1,
  2. DETLEV R. VOGLER2

Article first published online: 28 FEB 2002

DOI: 10.1046/j.1365-294x.1998.00409.x

Issue

Molecular Ecology

Molecular Ecology

Volume 7, Issue 7, pages 919–923, July 1998

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How to Cite

CULLINGS, K. W. and VOGLER, D. R. (1998), A 5.8S nuclear ribosomal RNA gene sequence database: applications to ecology and evolution. Molecular Ecology, 7: 919–923. doi: 10.1046/j.1365-294x.1998.00409.x

Author Information

  1. 1

    NASA-Ames Research Center, Building N239, MS 239–4, Moffett Field, California 94035–1000 USA,,

  2. 2

    Biology Department, San Francisco State University, 1600 Holloway Avenue, San Francsico, California 94132, USA

* K. W. Cullings Fax: +01-650-604-1088; E-mail: kcullings@mail.arc.nasa.gov

Publication History

  1. Issue published online: 28 FEB 2002
  2. Article first published online: 28 FEB 2002

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Keywords:

  • 5.8S rRNA;
  • DNA database;
  • DNA sequencing PCR

We compiled a 5.8S nuclear ribosomal gene sequence database for animals, plants, and fungi using both newly generated and GenBank sequences. We demonstrate the utility of this database as an internal check to determine whether the target organism and not a contaminant has been sequenced, as a diagnostic tool for ecologists and evolutionary biologists to determine the placement of asexual fungi within larger taxonomic groups, and as a tool to help identify fungi that form ectomycorrhizae.

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