Present address: Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ B5721, USA.
PCR amplification of the mating-type idiomorphs in Cryphonectria parasitica
Article first published online: 4 JAN 2002
Volume 8, Issue 11, pages 1947–1950, November 1999
How to Cite
Marra, R. E. and Milgroom, M. G. (1999), PCR amplification of the mating-type idiomorphs in Cryphonectria parasitica. Molecular Ecology, 8: 1947–1950. doi: 10.1046/j.1365-294x.1999.00783.x
- Issue published online: 4 JAN 2002
- Article first published online: 4 JAN 2002
- Received 8 April 1999; revision received 21 June 1999;accepted 21 June 1999
- Cryphonectria parasitica;
- mating type;
- mixed mating;
In the laboratory, the ascomycete fungus Cryphonectria parasitica is rarely self-fertile, and has a self-incompatibility system that resolves into two intersterility groups, controlled by a single locus. In natural populations, however, self-fertilization occurs frequently. In this report, we show that the C. parasitica self-incompatibility locus (MAT) comprises two idiomorphs (alleles that are highly divergent in sequence), conforming to the paradigm of self-incompatibility as described for other ascomycetes. Starting with a fragment putatively from the MAT-2 idiomorph, we used a PCR-based cloning approach to identify 3.5- and 2-kb sequences unique to MAT-1 and MAT-2 isolates, respectively. These sequences were then used to design idiomorph-specific PCR primer pairs, allowing us to efficiently identify the mating types of isolates, a crucial component of our research on the environmental and genetic factors underlying this mixed mating system.