Get access

Clonal structure in a dwarf bamboo (Sasa senanensis) population inferred from amplified fragment length polymorphism (AFLP) fingerprints

Authors


Yoshihisa Suyama.Present address: Department of Biodiversity Science, Tohoku University, Kawatabi, Naruko, Miyagi 989–6711 Japan. Fax: + 81 229 84 6490; E-mail:suyo@bios.tohoku.ac.jp

Abstract

Amplified fragment length polymorphism (AFLP) fingerprints were used to reveal clonal structure of a dense population of dwarf bamboo, Sasa senanensis, in a 10-ha study plot at Sugadaira Montane Research Center, University of Tsukuba, Nagano, Japan. We generated AFLP fingerprints for 51 leaf samples, collected at 50 m intervals, using three selective primer pairs. A total of 135–166 fragments were detected per sample, and 22 different fingerprints were identified based on 24–83 differing fragments. Our results demonstrate that the S. senanensis population in our plot consists of at least 22 clones and that the largest single clone occurs over a distance of about 300 m. Furthermore, the clone distribution pattern implies a relationship between site quality and clonal structure.

Ancillary