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Sigma-E is required for the production of the antibiotic actinomycin in Streptomyces antibioticus

  1. George H. Jones1,2,
  2. Mark S. B. Paget1,3,
  3. Leony Chamberlin1,3,
  4. Mark J. Buttner1,3

Article first published online: 31 OCT 2003

DOI: 10.1046/j.1365-2958.1997.2001566.x

Issue

Molecular Microbiology

Molecular Microbiology

Volume 23, Issue 1, pages 169–178, January 1997

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How to Cite

Jones, G. H., Paget, M. S. B., Chamberlin, L. and Buttner, M. J. (1997), Sigma-E is required for the production of the antibiotic actinomycin in Streptomyces antibioticus. Molecular Microbiology, 23: 169–178. doi: 10.1046/j.1365-2958.1997.2001566.x

Author Information

  1. 1

    Department of Genetics, John Innes Centre, Colney, Norwich NR4 7UH, UK.

  2. 2

    Department of Biology, Emory University, Atlanta, Georgia 30322, USA.

  3. 3

    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.

* Mark S. B. Paget E-mail PAGETM@BBSRC.AC.UK; Tel. (01603) 452571; Fax (01603) 456844.

Publication History

  1. Issue published online: 31 OCT 2003
  2. Article first published online: 31 OCT 2003

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The phsA gene encodes phenoxazinone synthase (PHS), which catalyses the penultimate step in the pathway for actinomycin biosynthesis in Streptomyces antibioticus. The phsA promoter strikingly resembles a putative StreptomycesσE cognate promoter, and purified EσE holoenzyme transcribed the phsA promoter in vitro. However, the phsA promoter was still active in an S. antibioticussigE null mutant and the level of PHS activity was unaffected. Despite this, disruption of sigE blocked actinomycin production completely. The loss of actinomycin production correlated with a 10-fold decrease in the activity of actinomycin synthetase I, the enzyme which catalyses the activation of the precursor of the actinomycin chromophore.

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