• 1
    Allaoui, A., Scheen, R., Lambert de Rouvroit, C.L., Cornelis, G.R. (1995a) VirG, a Yersinia enterocolitica lipoprotein involved in Ca2+ dependency, is related to ExsB of Pseudomonas aeruginosa. J Bacteriol 177: 42304237.
  • 2
    Allaoui, A., Schulte, R., Cornelis, G.R. (1995b) Mutational analysis of the Yersinia enterocolitica virC operon: characterization of yscE, F, G, I, J, K required for Yop secretion and yscH encoding YopR. Mol Microbiol 18: 343355.
  • 3
    Bergman, T., Håkansson, S., Forsberg, A., Norlander, L., Macellaro, A., Backman, A., et al (1991) Analysis of the V antigen lcrGVH-yopBD operon of Yersinia pseudotuberculosis: evidence for a regulatory role of LcrH and LcrV. J Bacteriol 173: 16071616.
  • 4
    Bhakdi, S., Mackman, N., Nicaud, J.M., Holland, I.B. (1986) Escherichia coli hemolysin may damage target cell membranes by generating transmembrane pores. Infect Immun 52: 6369.
  • 5
    Boland, A., Sory, M.-P., Iriarte, M., Kerbourch, C., Wattiau, P., Cornelis, G.R. (1996) Status of YopM and YopN in the Yersinia Yop virulon: YopM of Y. enterocolitica is internalized inside the cytosol of PU5-1.8 macrophages by the YopB, D, N delivery apparatus. EMBO J 15: 51915201.
  • 6
    Boyd, A.P., Sory, M.-P., Iriarte, M., Cornelis, G.R. (1998) Heparin interferes with translocation of Yop proteins into HeLa cells and binds to LcrG, a regulatory component of the Yersinia Yop apparatus. Mol Microbiol 27: 425436.
  • 7
    Cheng, L.W., Anderson, D.M., Schneewind, O. (1997) Two independent type III secretion mechanisms for YopE in Yersinia enterocolitica. Mol Microbiol 24: 757765.
  • 8
    China, B., Michiels, T., Cornelis, G.R. (1990) The pYV plasmid of Yersinia encodes a lipoprotein, YlpA, related to TraT. Mol Microbiol 4: 15851593.
  • 9
    Cornelis, G.R. & Colson, C. (1975) Restriction of DNA in Yersinia enterocolitica detected by recipient ability for a derepressed R factor from Escherichia coli. J Gen Microbiol 87: 285291.
  • 10
    Cornelis, G.R., Vanooteghem, J.C., Sluiters, C. (1987) Transcription of the yop regulon from Y. enterocolitica requires trans acting pYV and chromosomal genes. Microb Pathog 2: 367379.
  • 11
    Cornelis, G.R. & Wolf-Watz, H. (1997) The Yersinia Yop virulon: a bacterial system for subverting eukaryotic cells. Mol Microbiol 23: 861867.
  • 12
    Cornelis, G.R., Boland, A., Boyd, A.P., Geuijen, C., Iriarte, M., Neyt, C., et al (1998) The virulence plasmid of Yersinia, an antihost genome. Microbiol and Mol Biol Rev 62 (in press).
  • 13
    Eisenberg, D. (1984) Three-dimensional structure of membrane and surface proteins. Annu Rev Biochem 53: 595623.
  • 14
    Fields, K.A., Williams, A.W., Straley, S.C. (1997) Failure to detect binding of LcrH to the V antigen of Yersinia pestis. Infect Immun 65: 39543957.
  • 15
    Francis, M.S. & Wolf-Watz, H. (1998) YopD of Yersinia pseudotuberculosis is translocated into the cytosol of HeLa epithelial cells: evidence of a structural domain necessary for translocation. Mol Microbiol 29: 799813.
  • 16
    Frithz-Lindsten, E., Rosqvist, R., Johansson, L., Forsberg, Å . (1995) The chaperone-like protein YerA of Yersinia pseudotuberculosis stabilizes YopE in the cytoplasm but is dispensable for targeting to the secretion loci. Mol Microbiol 16: 635647.
  • 17
    Guan, K.L. & Dixon, J.E. (1991) Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal Biochem 192: 262267.
  • 18
    Håkansson, S., Bergman, T., Vanooteghem, J.-C., Cornelis, G., Wolf-Watz, H. (1993) YopB and YopD constitute a novel class of Yersinia Yop proteins. Infect Immun 61: 7180.
  • 19
    Håkansson, S., Galyov, E.E., Rosqvist, R., Wolf-Watz, H. (1996a) The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane. Mol Microbiol 20: 593603.
  • 20
    Håkansson, S., Schesser, K., Persson, C., Galyov, E.E., Rosqvist, R., Homblé, F., et al (1996b) The YopB protein of Yersinia pseudotuberculosis is essential for the translocation of Yop effector proteins across the target cell plasma membrane and displays a contact dependent membrane disrupting activity. EMBO J 15: 58125823.
  • 21
    Iriarte, M. & Cornelis, G.R. (1998) YopT, a new Yersinia Yop effector protein, affects the cytoskeleton of host cells. Mol Microbiol 29: 915929.
  • 22
    Iriarte, M., Sory, M.P., Boland, A., Boyd, A.P., Mills, S.D., Lambermont, I., et al (1998) TyeA, a protein involved in control of Yop release and in translocation of Yersinia Yop effectors. EMBO J 17: 19071918.
  • 23
    Kaniga, K., Delor, I., Cornelis, G.R. (1991) A wide-host-range suicide vector for improving reverse genetics in Gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica. Gene 109: 137141.
  • 24
    Kaniga, K., Tucker, S., Trollinger, D., Galan, J.E. (1995) Homologs of the Shigella IpaB and IpaC invasins are required for Salmonella entry into host cells. J Bacteriol 177: 39653971.
  • 25
    Khisty, V.J., Munske, G.R., Randall, L.L. (1995) Mapping of the binding frame for the chaperone SecB within a natural ligand, galactose-binding protein. J Biol Chem 270: 2592025927.
  • 26
    Koster, M., Bitter, W., De Cock, H., Allaoui, A., Cornelis, G.R., Tommassen, J. (1997) The outer membrane component, YscC, of the Yop secretion machinery of Yersinia enterocolitica forms a ring-shaped multimeric complex. Mol Microbiol 26: 789798.
  • 27
    Kumamoto, C.A. & Beckwith, J. (1985) Evidence for specificity at an early step in protein export in Escherichia coli. J Bacteriol 163: 267274.
  • 28
    Kunkel, T.A., Roberts, J.D., Zakour, R.A. (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol 154: 367382.
  • 29
    Lee, C.A. (1997) Type III secretion systems: machines to deliver bacterial proteins into eukaryotic cells? Trends Microbiol 5: 148156.
  • 30
    Liu, C.-C., Walsh, C.M., Young, J.D. (1995) Perforin: structure and function. Immunol Today 16: 194206.
  • 31
    Lupas, A., Van Dyke, M., Stock, J. (1991) Predicting coiled coils from protein sequences. Science 252: 11621164.
  • 32
    Ménard, R., Sansonetti, P.J., Parsot, C. (1993) Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells. J Bacteriol 175: 58995906.
  • 33
    Ménard, R., Sansonetti, P.J., Parsot, C., Vasselon, T. (1994) Extracellular association and cytoplasmic partitioning of the IpaB and IpaC invasins of S. flexneri. Cell 79: 515525.
  • 34
    Michiels, T., Wattiau, P., Brasseur, R., Ruysschaert, J.-M., Cornelis, G.R. (1990) Secretion of Yop proteins by Yersiniae. Infect Immun 58: 28402849.
  • 35
    Miller, V.L. & Mekalanos, J.J. (1988) A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol 170: 25752583.
  • 36
    Mills, S.D., Boland, A., Sory, M.-P., Van Der Smissen, P., Kerbourch, C., Finlay, B.B., et al (1997) Yersinia enterocolitica induces apoptosis in macrophages by a process requiring functional type III secretion and translocation mechanisms and involving YopP, presumably acting as an effector protein. Proc Natl Acad Sci USA 94: 1263812643.
  • 37
    Monack, D.M., Mecsas, J., Ghori, N., Falkow, S. (1997) Yersinia signals macrophages to undergo apoptosis and YopJ is necessary for this cell death. Proc Natl Acad Sci USA 94: 1038510390.
  • 38
    Mulder, B., Michiels, T., Simonet, M., Sory, M.-P., Cornelis, G.R. (1989) Identification of additional virulence determinants on the pYV plasmid of Yersinia enterocolitica W227. Infect Immun 57: 25342541.
  • 39
    Nilles, M.L., Fields, K.A., Straley, S.C. (1998) The V antigen of Yersinia pestis regulates Yop vectorial targeting as well as Yop secretion through effects on YopB and LcrG. J Bacteriol 180: 34103420.
  • 40
    Persson, C., Nordfelth, R., Holmström, A., Håkansson, S., Rosqvist, R., Wolf-Watz, H. (1995) Cell-surface-bound Yersinia translocate the protein tyrosine phosphatase YopH by a polarized mechanism into the target cell. Mol Microbiol 18: 135150.
  • 41
    Price, S.B. & Straley, S.C. (1989) lcrH, a gene necessary for virulence of Yersinia pestis and for the normal response of Y. pestis to ATP and calcium. Infect Immun 57: 14911498.
  • 42
    Price, S.B., Leung, K.Y., Barve, S.S., Straley, S.C. (1989) Molecular analysis of lcrGVH, the V antigen operon of Yersinia pestis. J Bacteriol 171: 56465653.
  • 43
    Rosqvist, R., Magnusson, K.-E., Wolf-Watz, H. (1994) Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells. EMBO J 13: 964972.
  • 44
    Ruckdeschel, K., Roggenkamp, A., Lafont, V., Mangeat, P., Heesemann, J., Rouot, B. (1997) Interaction of Yersinia enterocolitica with macrophages leads to macrophage cell death through apoptosis. Infect Immun 65: 48134821.
  • 45
    Sarker, M.R., Neyt, C., Stainier, I., Cornelis, G.R. (1998a) The Yersinia Yop virulon: LcrV is required for extrusion of the translocators YopB and YopD. J Bacteriol 180: 12071214.
  • 46
    Sarker, M.R., Sory, M.-P., Boyd, A.P., Iriarte, M., Cornelis, G.R. (1998b) LcrG is required for efficient internalization of Yersinia Yop effector proteins into eukaryotic cells. Infect Immun 66: 29762979.
  • 47
    Schesser, K., Frithz-Lindsten, E., Wolf-Watz, H. (1996) Delineation and mutational analysis of the Yersinia pseudotuberculosis YopE segments which mediate translocation across bacterial and eukaryotic cellular membranes. J Bacteriol 178: 72277233.
  • 48
    Schesser, K., Spiik, A.K., Dukuzumuremyi, J.M., Neurath, M.F., Pettersson, S., Wolf-Watz, H. (1998) The yopJ locus is required for Yersinia-mediated inhibition of NF-κB activation and cytokine expression: YopJ contains a eukaryotic SH2-like domain that is essential for its repressive activity. Mol Microbiol 28: 10671079.
  • 49
    Skrzypek, E. & Straley, S.C. (1993) LcrG, a secreted protein involved in negative regulation of the low-calcium response in Yersinia pestis. J Bacteriol 175: 35203528.
  • 50
    Smith, D.B. & Johnson, K.S. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67: 3140.
  • 51
    Sory, M.-P. & Cornelis, G.R. (1994) Translocation of a hybrid YopE-adenylate cyclase from Yersinia enterocolitica into HeLa cells. Mol Microbiol 14: 583594.
  • 52
    Sory, M.-P., Boland, A., Lambermont, I., Cornelis, G.R. (1995) Identification of the YopE and YopH segments required for secretion and internalization into the cytosol of macrophages, using the cyaA gene fusion approach. Proc Natl Acad Sci USA 92: 1199812002.
  • 53
    Straley, S.C. & Perry, R.D. (1995) Environmental modulation of gene expression and pathogenesis in Yersinia. Trends Microbiol 3: 310317.
  • 54
    Strathdee, C.A. & Lo, R.Y. (1989) Cloning, nucleotide sequence, and characterization of genes encoding the secretion function of the Pasteurella haemolytica leukotoxin determinant. J Bacteriol 171: 916928.
  • 55
    Tabor, S. & Richardson, C.C. (1985) A bacteriophage T7 RNA polymerase promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 82: 10741078.
  • 56
    Wainwright, L.A. & Kaper, J.B. (1998) EspB and EspD require a specific chaperone for proper secretion from enteropathogenic Escherichia coli. Mol Microbiol 27: 12471260.
  • 57
    Wattiau, P. & Cornelis, G.R. (1993) SycE, a chaperone-like protein of Yersinia enterocolitica involved in the secretion of YopE. Mol Microbiol 8: 123131.
  • 58
    Wattiau, P., Bernier, B., Deslee, P., Michiels, T., Cornelis, G.R. (1994) Individual chaperones required for Yop secretion by Yersinia. Proc Natl Acad Sci USA 91: 1049310497.
  • 59
    Wattiau, P., Woestyn, S., Cornelis, G.R. (1996) Customized secretion chaperones in pathogenic bacteria. Mol Microbiol 20: 255262.
  • 60
    Williams, A.W. & Straley, S.C. (1998) YopD of Yersinia pestis plays a role in negative regulation of the low-calcium response in addition to its role in translocation of Yops. J Bacteriol 180: 350358.
  • 61
    Woestyn, S., Sory, M.-P., Boland, A., Lequenne, O., Cornelis, G.R. (1996) The cytosolic SycE and SycH chaperones of Yersinia protect the region of YopE and YopH involved in translocation across eukaryotic cell membranes. Mol Microbiol 20: 12611271.
  • 62
    Yahr, T.L., Mende-Mueller, L.M., Friese, M.B., Frank, D.W. (1997) Identification of type III secreted products of the Pseudomonas aeruginosa exoenzyme S regulon. J Bacteriol 179: 71657168.