• 1
    App, H. & Holzer, H. (1989)Purification and characterisation of neutral trehalase from yeast ABYS1 mutant. J Biol Chem 263: 1758317588.
  • 2
    Bell, W., Klaassen, P., Ohnacker, M., Boller, T., Herweijer, M., Schoppink, P., et al. (1992) Characterization of the 56kDa subunit of the yeast trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation. Eur J Biochem 209: 951959.
  • 3
    Bell, W., Sun, W., Hohmann, S., Wera, S., Reinders, A., De Virgilio, C., et al. (1998) Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex. J Biol Chem 273: 3331133319.
  • 4
    Blomberg, A. & Adler, L. (1992) Physiology of osmotolerance in fungi. Adv Microbiol Phys 33: 145212.
  • 5
    Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal Biochem 72: 248254.
  • 6
    De Virgilio, C., Bürckert, N., Boller, T., Wiemken, A. (1991) A method to study the rapid phosphorylation-related modulation of neutral trehalase activity by temperature shifts in yeast. FEBS Lett 291: 355358.
  • 7
    De Virgilio, C., Bürckert, N., Bell, W., Jeno, P., Boller, T., Wiemken, A. (1993) Disruption of TPS2, the gene encoding the 100kDa-subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity. Eur J Biochem 212: 315323.
  • 8
    Degols, G., Shiozaki, K., Russel, P. (1996) Activation and regulation of the Spc1 stress-activated protein kinase in Schizosaccharomyces pombe. Mol Cell Biol 16: 28702877.
  • 9
    DeRisi, J.L., Iyer, V.R., Brown, P.O. (1997) Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278: 680686.
  • 10
    Elder, R.T., Loy, E.Y., Davis, R.W. (1983) RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA. Proc Natl Acad Sci USA 8: 24323436.
  • 11
    Fernandez, J., Soto, T., Vicente-Soler, J., Cansado, J., Gacto, M. (1997) Osmo-stress induced changes in neutral trehalase activity in the fission yeast Schizosaccharomyces pombe. Biochim Biophys Acta 1357: 4148.
  • 12
    Gallwitz, D. & Sures, I. (1980) Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 77: 25462550.
  • 13
    Garcia, J.M., Rios, G., Ali, R., Belles, J.M., Serrano, R. (1997) Comparative physiology of salt tolerance in Candida tropicalis and Saccharomyces cerevisiae. Microbiology 143: 11251131.
  • 14
    Gietz, R.D. & Schiestl, R.H. (1995) Transforming yeast with DNA. Methods Mol Cell Biol 5: 255269.
  • 15
    Görner, W., Durchschlag, E., Martinez-Pastor, T.M., Estruch, F., Ammerer, G., Hamilton, B., et al. (1998) Nuclear localization of the C2H2 zinc finger protein Msn2p is regulated by stress and protein kinase A activity. Genes Dev 12: 586597.
  • 16
    Gounalaki, N. & Thireos, G. (1994) Yap1, a yeast transcriptional activator that mediates multidrug resistance, regulates the metabolic stress response. EMBO J 13: 40364041.
  • 17
    Herrick, D., Parker, R., Jacobson, A. (1990) Identification and comparison of stable and unstable mRNAs in S. cerevisiae. Mol Cell Biol 10: 22692284.
  • 18
    Kienle, I., Burgert, M., Holzer, H. (1993) Assay of trehalose with acid trehalase purified from Saccharomyces cerevisiae. Yeast 9: 607611.
  • 19
    Kobayashi, N. & McEntee, K. (1993) Identification of cis and trans components of a novel heat shock regulatory pathway in Saccharomyces cerevisiae. Mol Cell Biol 13: 248256.
  • 20
    Kopp, M., Müller, H., Holzer, H. (1993) Molecular analysis of the neutral trehalase gene from Saccharomyces cerevisiae. J Biol Chem 268: 47664774.
  • 21
    Kopp, M., Nwaka, S., Holzer, H. (1994) Corrected sequence of the neutral trehalase encoding gene NTH1: biological implications. Gene 150: 403404.
  • 22
    Lewis, J.G., Learmonth, R.P., Watson, K. (1995) Induction of heat, freezing and salt tolerance by heat and salt shock in Saccharomyces cerevisiae. Microbiology 141: 687694.
  • 23
    Marchler, G., Schuller, C., Adam, G., Ruis, H. (1993) A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J 12: 19972003.
  • 24
    Marquez, J.A., Pascual-Ahuir, A., Proft, M., Serrano, R. (1998) The Ssn6–Tup1 repressor complex of Saccharomyces cerevisiae is involved in the osmotic induction of HOG-dependent and -independent genes. EMBO J 17: 25432553.
  • 25
    Martinez-Pastor, M.T., Marchler, G., Schüller, C., Marchler-Bauer, A., Ruis, H., Estruch, F. (1996) The Saccharomyces cerevisiae zinc finger protein Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). EMBO J 15: 22272235.
  • 26
    Mazon, M.J., Behrens, M.M., Morgado, E., Portilo, F. (1993) Low activity of the yeast cAMP-dependent protein kinase catalytic subunit Tpk3 due to the poor expression of the TPK3 gene. Eur J Biochem 213: 501506.
  • 27
    Mesquita, J.F., Paschoalin, V.M.F., Panek, A.D. (1997) Modulation of trehalase activity in Saccharomyces cerevisiae by an intrinsic protein. Biochim Biophys Acta 1334: 233239.
  • 28
    Mikaelian, I. & Sergeant, A. (1992) A general and fast method to generate multiple site directed mutations. Nucleic Acids Res 20: 376.
  • 29
    Moskvina, E., Schüller, C., Maurer, C.T.C., Mager, W.H., Ruis, H. (1998) A search in the genome of Saccharomyces cerevisiae for genes regulated via stress response elements. Yeast 14: 10411050.DOI: 10.1002/(sici)1097-0061(199808)14:11<1041::aid-yea296>;2-4
  • 30
    Myers, A.M., Tzagoloff, A., Kinney, D.M., Lusty, C. (1986) Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299310.
  • 31
    Nikawa, J., Cameron, S., Toda, T., Ferguson, K.W., Wigler, M. (1987) Rigorous feedback control of cAMP levels in Saccharomyces cerevisiae. Genes Dev 1: 931937.
  • 32
    Nwaka, S., Kopp, M., Holzer, H. (1995a) Expression and function of the trehalase genes NTH1 and YBR106 in Saccharomyces cerevisiae. J Biol Chem 270: 1019310198.
  • 33
    Nwaka, S., Mechler, B., Destruelle, M., Holzer, H. (1995b) Phenotypic features of trehalase mutants in Saccharomyces cerevisiae. FEBS Lett 360: 286290.DOI: 10.1016/0014-5793(95)00105-i
  • 34
    Nwaka, S. & Holzer, H. (1998) Molecular biology of trehalose and the trehalases in the yeast Saccharomyces cerevisiae. In Progress in Nucleic Acid Research and Molecular Biology, vol. 58. Moldave, K. (ed.). San Diego: Academic Press, pp. 197237.
  • 35
    Parrou, J.L., Teste, M.-A., Francois, J. (1997) Effects of various types of stress on the metabolism of reserve carbohydrate in Saccharomyces cerevisiae: genetic evidence for stress-induced recycling of glycogen and trehalose. Microbiology 143: 18911900.
  • 36
    Parrou, J.L., Enjalbert, B., Plourde, L., Bauche, A., Gonzales, B., Francois, J. (1999) Dynamic responses of reserve carbohydrate metabolism under carbon and nitrogen limitations in Saccharomyces cerevisiae. Yeast 15: 191203.DOI: 10.1002/(sici)1097-0061(199902)15:3<191::aid-yea358>;2-o
  • 37
    Piper, P.W. (1993) Molecular events associated with acquisition of heat tolerance by the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 11: 339356.
  • 38
    Piper, P.W. (1998) Differential role of Hsps and trehalose in stress tolerance. Trends Microbiol 6: 4344.DOI: 10.1016/s0966-842x(97)01190-6
  • 39
    Praekelt, U.M. & Meacock, P.A. (1990) HSP12, a new small heat shock gene of Saccharomyces cerevisiae. Analysis of structure, regulation and function. Mol Gen Genet 223: 97106.
  • 40
    Rose, M.D., Winston, F., Hieter, P. (1990) Methods in Yeast Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  • 41
    Rothstein, R.J. (1991) Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol 194: 202211.
  • 42
    Sambrook, J., Fritsch, E.F., Maniatis, T. (1989) Molecular Cloning: A laboratory Manual. Cold Spring Harbor Laboratory Press, NY: Cold Spring Harbor Laboratory Press.
  • 43
    San Miguel, P.F. & Arguelles, J.C. (1994) Differential changes in the activity of cytosolic and vacuolar trehalases along the growth cycle of Saccharomyces cerevisiae. Biochim Biophys Acta 1200: 155160.
  • 44
    Schmitt, A.P. & McEntee, K. (1996) Msn2p, a zinc finger DNA-binding protein, is the transcriptional activator of the multistress response in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 93: 57775782.
  • 45
    Sherman, F. (1991) Getting started with yeast. Methods Enzymol 194: 321.
  • 46
    Siderius, M. & Mager, W.H. (1997) General stress response: in search of a common denominator. In Yeast Stress Responses. Hohmann, S., and Mager, W.H. (eds). Heidelberg: Springer-Verlag, pp. 213230.
  • 47
    Singer, M. & Lindquist, S. (1998) Multiple effects of trehalose on protein folding in-vitro and in-vivo. Mol Cell 1: 639648.
  • 48
    Soto, T., Fernandez, J., Dominguez, A., Vicente-Soler, J., Cansado, J., Gacto, M. (1998) Analysis of the ntp1+ gene, encoding neutral trehalase in the fission yeast Schizosaccharomyces pombe. Biochim Biophys Acta 1443: 225229.
  • 49
    Thevelein, J.M. (1984) Regulation of trehalose mobilization in fungi. Microbiol Rev 48: 4259.
  • 50
    Thevelein, J.M. (1988) Regulation of trehalase activity by phosphorylation–dephosphorylation during developmental transition in fungi. Exp Mycol 12: 17.
  • 51
    Thevelein, J.M. (1996) Regulation of trehalose metabolism and its relevance to cell growth and function. In The Mycota III, Biochemistry and Molecular Biology. Brambl, R., and Marzluf, G.A. (eds). Heidelberg: Springer-Verlag, pp. 395414.
  • 52
    Thevelein, J.M. & Beullens, M. (1985) Cyclic AMP and the stimulation of trehalase activity in the yeast Saccharomyces cerevisiae by carbon sources, nitrogen sources and inhibitors of protein synthesis. J Gen Microbiol 131: 31993209.
  • 53
    Toda, T., Cameron, S., Sass, P., Zoller, M., Wigler, M. (1987) Three different genes in Saccharomyces cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell 50: 277287.
  • 54
    Uno, I., Matsumoto, K., Adachi, K., Ishikawa, T. (1983) Genetic and biochemical evidence that trehalase is a substrate of cAMP-dependent protein kinase in yeast. J Biol Chem 258: 1086710872.
  • 55
    Van Dijck, P., Colavizza, D., Smet, P., Thevelein, J. (1995) Differential importance of trehalose in stress resistance in fermenting and nonfermenting Saccharomyces cerevisiae cells. Appl Environ Microbiol 61: 109115.
  • 56
    Werner, W., Rey, H.-G., Wielinger, H. (1970) On the properties of a new chromogen for the determination of glucose in blood according to GOD/POD-method. Z Anal Chem 252: 224226.
  • 57
    Wiemken, A. (1990) Trehalose in yeast, stress protectant rather than reserve carbohydrate. Antonie Van Leeuwenhoek 58: 209217.
  • 58
    Winderickx, J., De Winde, J.H., Crauwels, M., Hino, A., Hohmann, S., Van Dijck, P., Thevelein, J.M. (1996) Regulation of genes encoding subunits of the trehalose synthase complex in Saccharomyces cerevisiae: novel variations of STRE-mediated transcriptional control? Mol Gen Genet 252: 470482.DOI: 10.1007/s004380050252
  • 59
    Zähringer, H. (1999) Regulation und funktion der neutralen trehalase bei der reaktion der hefe Saccaromyces cerevisiae auf globalen stress. PhD thesis. Freiburg, Germany: University of Freiburg.
  • 60
    Zähringer, H., Burgert, M., Holzer, H., Nwaka, S. (1997) Neutral trehalase Nth1p of Saccharomyces cerevisiae encoded by the NTH1 gene is a multiple stress responsive protein. FEBS Lett 412: 615620.DOI: 10.1016/s0014-5793(97)00868-5
  • 61
    Zähringer, H., Holzer, H., Nwaka, S. (1998) Stability of neutral trehalase during heat stress in Saccharomyces cerevisiae is dependent on the catalytic subunits of cAMP-dependent protein kinase, Tpk1 and Tpk2. Eur J Biochem 255: 544551.