• 1
    Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990) A basic local alignment search tool. J Mol Biol 215: 403410.
  • 2
    Amerik, A.Y., Antonov, W.K., Gobalenya, A.E., Kotova, S.A., Rotanova, T.V., and Shimbarevich, E.V. (1991) Site directed mutagenesis of La protease: a catalytically active residue. FEBS Lett 287: 211214.
  • 3
    Baldwin, C.L., and Winter, A.J. (1994) Macrophage–Pathogen Interactions. Zwilling, B.S., and Eisenstein, T.K. (eds). New York: Marcel Dekker, pp. 363380.
  • 4
    Brill, J.A., Quinlan-Walshe, C., and Gottesman, S. (1988) Fine-structure mapping and identification of two regulators of capsule synthesis in Escherichia coli K-12. J Bacteriol 170: 25992611.
  • 5
    Buchmeier, N.A., and Heffron, F. (1990) Induction of Salmonella stress proteins upon infection of macrophages. Science 248: 730732.
  • 6
    Canning, P.C. (1990) Phagocyte function in resistance to brucellosis. In Advances in Brucellosis Research. Adams, L.G. (ed.). College Station, TX: Texas A & M University Press, pp. 151163.
  • 7
    Cellier, M.F.M., Teyssier, J., Nichols, M., Liautard, J.P., Marti, J., and Sri Widada, J. (1992) Cloning and characterization of the Brucella ovis heat shock protein DnaK functionally expressed in Escherichia coli. J Bacteriol 174: 80368042.
  • 8
    Chin, D.T., Goff, S.A., Webster, T., Smith, T., and Goldberg, A.L. (1988) Sequence of the lon gene in Escherichia coli. J Biol Chem 263: 1171811728.
  • 9
    Chung, C.H. (1993) Proteases of Escherichia coli. Science 262: 372374.
  • 10
    Comczynski, P., and Sacchi, M. (1987) Single-step method of RNA isolation by acid guanidium thiocyanate–phenol–chloroform extraction. Anal Biochem 112: 156.
  • 11
    Davies, K.J.A., and Lin, S.W. (1988) Oxidatively denatured proteins are degraded by an ATP-independent proteolytic pathway in Escherichia coli. Free Radical Biol Med 5: 225236.
  • 12
    Eastgate, J.A., Taylor, N., Coleman, M.J., Healy, B., Thompson, L., and Roberts, I.S. (1995) Cloning, expression, and characterization of the lon gene of Erwinia amylovora: evidence for heat shock response. J Bacteriol 177: 932937.
  • 13
    Elzer, P.H., Phillips, R.W., Kovach, M.E., Peterson K.M., and Roop, II, R.M. (1994) Characterization and genetic complementation of a Brucella abortus high-temperature-requirement A (htrA) deletion mutant. Infect Immun 62: 41354139.
  • 14
    Elzer, P.H., Phillips, R.W., Robertson G.T., and Roop, II, R.M. (1996) The HtrA stress response protease contributes to resistance of Brucella abortus to killing by murine phagocytes. Infect Immun 64: 48384841.
  • 15
    Fischer, H., and Glockshuber, R. (1994) A point mutation within the ATP-binding site inactivates both catalytic functions of the ATP-dependent protease La (Lon) from Escherichia coli. FEBS Lett 356: 101103.
  • 16
    Garrido, T., Sanchez, M., Palacios, P., Aldea, M., and Vicente, M. (1993) Transcription of ftsZ oscillates during the cell cycle of Escherichia coli. EMBO J 12: 39573965.
  • 17
    Goldberg, A.L. (1972) Degradation of abnormal proteins in Escherichia coli. Proc Natl Acad Sci USA 69: 422426.
  • 18
    Gottesman, S. (1996) Proteases and their targets in Escherichia coli. Annu Rev Genet 30: 465506.
  • 19
    Gottesman, S., and Maurizi, M.R. (1992) Regulation by proteolysis: energy-dependent proteases and their targets. Microbiol Rev 56: 592621.
  • 20
    Gottesman, S., Clark, W.P., De Crecy-Lagard, V., and Maurizi, M.R. (1993) ClpX, an alternate subunit for the ATP-dependent Clp protease of Escherichia coli. Sequence and in vivo activities. J Biol Chem 268: 2261822626.
  • 21
    Kano, Y., Yoshino, S., Wada, M., Yokoyama, K., Nobuhara, M., and Imamoto, F. (1985) Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli. Mol Gen Genet 201: 360362.
  • 22
    Köhler, S., Teyssier, J., Cloeckaert, A., Rouot, B., and Liautard, J.P. (1996) Participation of the molecular chaperone DnaK in intracellular growth of Brucella suis within U937-derived phagocytes. Mol Microbiol 20: 701712.
  • 23
    Kovach, M.E., Phillips, R.W., Elzer, P.H., and Roop, II, R.M., and Peterson, K.M. (1994) pBBR1MCS: a broad-host-range cloning vector. Biotechnology 16: 800.
  • 24
    Laskowska, E., Kuczyska-Winik, D., Skórko-Glonek, J., and Taylor, A. (1996) Degradation by proteases Lon, Clp and HtrA, of Escherichia coli proteins aggregated in vivo by heat shock; HtrA protease action in vivo and in vitro. Mol Microbiol 22: 555571.
  • 25
    Latch, J.N., and Margolin, W. (1997) Generation of buds, swelling, and branches instead of filaments after blocking the cell cycle of Rhizobium meliloti. J Bacteriol 179: 23732381.
  • 26
    Lin, J., and Ficht, T. (1995) Protein synthesis in Brucella abortus induced during macrophage infection. Infect Immun 63: 14091414.
  • 27
    Lin, J., Adams, L.G., and Ficht, T.A. (1992) Characterization of the heat shock response in Brucella abortus and isolation of the genes encoding the GroE heat shock proteins. Infect Immun 60: 24252431.
  • 28
    López-Gon~i, I., Moriyón, I., and Neilands, J.B. (1992) Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore. Infect Immun 60: 44964503.
  • 29
    Maurizi, M.R. (1992) Proteases and protein degradation in Escherichia coli. Experientia 48: 178200.
  • 30
    Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  • 31
    Mori, E., Fulchieri, M., Indorato, C., Fani, R., and Bazzicalupo, M. (1996) Cloning, nucleotide sequencing, and expression of the Azospirillum brasilense lon gene: involvement in iron uptake. J Bacteriol 178: 34403446.
  • 32
    Nicoletti, P. (1989) Brucellosis: Clinical and Laboratory Aspects. Young, E.J., and Corbel, M.J. (eds). Boca Raton, FL: CRC Press, pp. 4152.
  • 33
    Parsell, D.A., and Lindquist, S. (1993) The function of heat-shock proteins in stress tolerance: Degradation and reactivation of damaged proteins. Annu Rev Genet 27: 437496.
  • 34
    Phillips, R.W., Elzer, P.H., Robertson, G.T., Hagius, S.D., Walker, J.V., Fatemi, M.B., et al (1997) A Brucella melitensis high temperature requirement A (htrA) deletion mutant is attenuated in goats and protects against abortion. Res Vet Sci 63: 165167.
  • 35
    Phillips, T.A., Van Bogelen, R.A., and Neidhardt, F.C. (1984) lon gene product of Escherichia coli is a heat shock protein. J Bacteriol 159: 283287.
  • 36
    Rafie-Kolpin, M., Essenberg R.C., and Wyckoff, III, J.H. (1996) Identification and comparison of macrophage-induced proteins and proteins induced under various stress conditions in Brucella abortus. Infect Immun 64: 52745283.
  • 37
    Robertson, G.T., and Roop, II, R.M. (1999) The Brucella abortus host factor I (HF-I) protein contributes to stress resistance during stationary phase and is a major determinant of virulence in mice. Mol Microbiol 34: 690700.
  • 38
    Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  • 39
    Sanger, F., Nickelen, S., and Coulson, R. (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 54635467.
  • 40
    Schmidt, R., Decatur, A.L., Rather, P.N., and Moran, Jr, C.P., and Losick, R. (1994) Bacillus subtilis Lon protease prevents inappropriate transcription of genes under control of the sporulation transcription factor σG. J Bacteriol 176: 65286537.
  • 41
    Schoemaker, J.M., Randall, C.G., and Markovitz, A. (1984) Regulation of cell division in Escherichia coli: SOS induction and cellular location of the SulA protein, a key to lon-associated filamentation and death. J Bacteriol 158: 551561.
  • 42
    Sha, Z. (1994) Purification, characterization and cloning of a periplasmic catalase from B. abortus and the role it plays in the pathogenesis of Brucella. PhD Thesis, Ames, IA: Department of Zoology and Genetics, Iowa State University.
  • 43
    Snedecor, G.W., and Cochran, W.G. (1989) Statistical Methods, 8th edn. Ames, IA: Iowa State University Press.
  • 44
    Sonezaki, S., Kondo, A., Oba, T., Ishii, Y., Kato, Y., and Nakayama, H. (1994) Overproduction and purification of the Lon protease from Escherichia coli using a maltose-binding protein fusion system. Appl Microbiol Biotechnol 42: 313318.
  • 45
    Stephens, C., Reisenauer, A., Wright, R., and Shapiro, L. (1996) A cell cycle-regulated bacterial DNA methyltransferase is essential for viability. Proc Natl Acad Sci USA 93: 12101214.
  • 46
    Stewart, B.J., Enos-Berlage, J.L., and McCarter, L.L. (1997) The lonS gene regulates swarmer cell differentiation of Vibrio parahaemolyticus. J Bacteriol 179: 107114.
  • 47
    Tatum, F.M., Morfitt, D.C., and Halling, S.M. (1993) Construction of a Brucella abortus RecA mutant and its survival in mice. Microb Pathog 14: 177185.
  • 48
    Tatum, F.M., Cheville, N.F., and Morfitt, D. (1994) Cloning, characterization and construction of htrA and htrA-like mutants of Brucella abortus and their survival in BALB/c mice. Microb Pathog 17: 2336.
  • 49
    Tojo, N., Inouye, S., and Komano, T. (1993) The lonD gene is homologous to the lon gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus. J Bacteriol 175: 45454549.
  • 50
    Torres-Cabassa, A., and Gottesman, S. (1987) Capsule synthesis in Escherichia coli K-12 is regulated by proteolysis. J Bacteriol 169: 981989.
  • 51
    Wright, R., Stephens, C., Zweiger, G., Shapiro, L., and Alley, M.R.K. (1996) Caulobacter Lon protease has a critical role in cell-cycle control of DNA methylation. Genes Dev 10: 15321542.
  • 52
    Wright, R., Stephens, C., and Shapiro, L. (1997) The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus. J Bacteriol 179: 58695877.
  • 53
    Zhu, Y., Oliveira, S.C., and Splitter, G.A. (1993) Isolation of Brucella abortus ssb and uvrA genes from a genomic library by use of lymphocytes as probes. Infect Immun 61: 53395344.