MtrC, an outer membrane decahaem c cytochrome required for metal reduction in Shewanella putrefaciens MR-1
Article first published online: 21 DEC 2001
Volume 39, Issue 3, pages 722–730, February 2001
How to Cite
Beliaev, A. S., Saffarini, D. A., McLaughlin, J. L. and Hunnicutt, D. (2001), MtrC, an outer membrane decahaem c cytochrome required for metal reduction in Shewanella putrefaciens MR-1. Molecular Microbiology, 39: 722–730. doi: 10.1046/j.1365-2958.2001.02257.x
- Issue published online: 21 DEC 2001
- Article first published online: 21 DEC 2001
- Accepted 23 October, 2000.
Shewanella putrefaciens is a facultative anaerobe that can use metal oxides as terminal electron acceptors during anaerobic respiration. Two proteins, MtrB and Cct, have been identified that are specifically involved in metal reduction. Analysis of S. putrefaciens mutants deficient in metal reduction led to the identification of two additional proteins that are involved in this process. MtrA is a periplasmic decahaem c-type cytochrome that appears to be part of the electron transport chain, which leads to Fe(III) and Mn(IV) reduction. MtrC is an outer membrane decahaem c-type cytochrome that appears to be required for the activity of the terminal Fe(III) reductase. Membrane fractions of mutants deficient in MtrC exhibited a decreased level of Fe(III) reduction compared with the wild type. We suggest that MtrC may be a component of the terminal reductase or may be required for its assembly.