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Immediate GTP hydrolysis upon FtsZ polymerization
Article first published online: 25 APR 2002
Volume 43, Issue 6, pages 1517–1521, March 2002
How to Cite
Scheffers, D.-J. and Driessen, A. J. M. (2002), Immediate GTP hydrolysis upon FtsZ polymerization. Molecular Microbiology, 43: 1517–1521. doi: 10.1046/j.1365-2958.2002.02828.x
- Issue published online: 25 APR 2002
- Article first published online: 25 APR 2002
To understand the polymerization dynamics of FtsZ, a bacterial cell division protein similar to tubulin, insight is required into the nature of the nucleotide bound to the polymerized protein. In a previous study, we showed that the FtsZ polymers contain mostly GDP. A recent study challenged this result, suggesting that the polymerized FtsZ is in a GTP-bound state. Here, we show that, when radiolabelled [γ-32P]-GTP is used to polymerize FtsZ, GTP is hydrolysed instantaneously. The FtsZ polymer contains both GDP and the radiolabelled inorganic phosphate.