1365-2958/asset/olbannerleft.gif?v=1&s=5badf94c134fc8798f790efd4821c55ff95fc4cb)
A site-specific recombinase (RinQ) is required to exert incompatibility towards the symbiotic plasmid of Rhizobium etli
Article first published online: 6 NOV 2002
DOI: 10.1046/j.1365-2958.2002.03205.x
1365-2958/asset/cover.gif?v=1&s=75254d9e3ae6a0f08085f34cc6e5bd65d8e3d52e "Molecular Microbiology")
Additional Information
How to Cite
Quintero, V., Cevallos, M. A. and Dávila, G. (2002), A site-specific recombinase (RinQ) is required to exert incompatibility towards the symbiotic plasmid of Rhizobium etli. Molecular Microbiology, 46: 1023–1032. doi: 10.1046/j.1365-2958.2002.03205.x
Publication History
- Issue published online: 6 NOV 2002
- Article first published online: 6 NOV 2002
- Accepted 5 August, 2002.
Summary
The replication/partition region of the symbiotic plasmid p42d of Rhizobium etli CE3 is characterized by the presence of the repABC operon. A recombinant plasmid containing this region is able to replicate in a R. etli derivative cured from p42d, with the same stability and copy number shown by the parental plasmid. However, when this construct is introduced into the wild-type strain, instead of exerting incompatibility against the p42d, it forms a stable cointegrate with it. In this paper, we show that a site-specific resolvase, and its action sites are essential factors to displace the symbiotic p42d. We propose a model for this novel incompatibility mechanism.