Cell wall α-1,3-glucan is required to anchor the Cryptococcus neoformans capsule
Article first published online: 7 NOV 2003
Volume 50, Issue 4, pages 1401–1409, November 2003
How to Cite
Reese, A. J. and Doering, T. L. (2003), Cell wall α-1,3-glucan is required to anchor the Cryptococcus neoformans capsule. Molecular Microbiology, 50: 1401–1409. doi: 10.1046/j.1365-2958.2003.03780.x
- Issue published online: 7 NOV 2003
- Article first published online: 7 NOV 2003
- Accepted 18 August, 2003.
Cryptococcus neoformans is an opportunistic pathogen responsible for serious disease in humans. Critical for virulence of this fungus is an elaborate polysaccharide capsule, which impedes the host immune response. We found that association of the capsule with the cell requires a specific component of the cell wall, α-1,3-glucan. Post-transcriptional inhibition of α-1,3-glucan synthase expression, using double-stranded RNA interference, yields cells that are unable to assemble a capsule although they generate its polysaccharide components. The resulting cryptococci are slow-growing and acapsular. This finding demonstrates a novel mode of polysaccharide attachment and an important application of RNA interference in fungi. The elimination of the capsule by reducing the expression of a single gene suggests a potential avenue for antifungal chemotherapy.