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Differential expression of three members of the AMT1 gene family encoding putative high-affinity NH4+ transporters in roots of Oryza sativa subspecies indica

  1. A. KUMAR1,
  2. S. N. SILIM2,
  3. M. OKAMOTO3,
  4. M. Y. SIDDIQI1,
  5. A. D. M. GLASS1,*

Article first published online: 9 JUN 2003

DOI: 10.1046/j.1365-3040.2003.01023.x

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Plant, Cell & Environment

Plant, Cell & Environment

Volume 26, Issue 6, pages 907–914, June 2003

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How to Cite

KUMAR, A., SILIM, S. N., OKAMOTO, M., SIDDIQI, M. Y. and GLASS, A. D. M. (2003), Differential expression of three members of the AMT1 gene family encoding putative high-affinity NH4+ transporters in roots of Oryza sativa subspecies indica. Plant, Cell & Environment, 26: 907–914. doi: 10.1046/j.1365-3040.2003.01023.x

Author Information

  1. 1

    Department of Botany, the University of British Columbia, Vancouver, BC, Canada V6T 1Z4,

  2. 2

    Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada S0G 2K0 and

  3. 3

    Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA

* Anthony D. M. Glass. Fax: +1 604 822 6089; e-mail: aglass@interchange.ubc.ca

Publication History

  1. Issue published online: 9 JUN 2003
  2. Article first published online: 9 JUN 2003

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REFERENCES

  • Bagchi S.N., Rai U.N., Rai A.N. & Singh H.N. (1985) Nitrate metabolism in the cyanobacterium Anabaena cycadeae: regulation of nitrate uptake and reductase by ammonia. Physiologia Plantarum 63, 322–326.
    Direct Link:
  • Becking J.H. (1956) On the mechanism of ammonium uptake by maize roots. Acta Botanica Neerlandica 5, 2–79.
  • Cooper H.D. & Clarkson D.T. (1989) Cycling of amino-nitrogen and other nutrients between shoots and roots in cereals – a possible mechanism integrating shoot and root in the regulation of nutrient uptake. Journal of Experimental Botany 40, 753–762.
  • Cserzo M., Wallin E., Simon I., Von Heijne G. & Elofsson A. (1997) Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the Dense Alignment Surface method. Protein Engineering 10, 673–676.
  • Dubois E. & Grenson M. (1979) Methylamine/ammonia uptake system in Saccharomyces cerevisiae: multiplicity and regulation. Molecular and General Genetics 175, 67–76.
  • Feng J., Volk R.J. & Jackson W.A. (1994) Inward and outward transport of ammonium in roots of maize and sorghum: contrasting effects of methionine sulfoximine. Journal of Experimental Botany 45, 429–439.
  • Fentem P.A., Lea P.J. & Stewart G.R. (1983) Ammonia assimilation in the roots of nitrate and ammonia grown Hordeum vulgare L. (cv. Golden promise). Plant Physiology 71, 496–501.
  • Flores E., Guerrero M.G. & Losada M. (1980) Short term ammonium inhibition of nitrate utilization by Anacystis nidulans and other cyanobacteria. Archives of Microbiology 128, 137–144.
  • Gazzarrini S., Lejay L., Gojon A., Ninnemann O. & Frommer W.B. (1999) Three functional transporters for constitutive, diurnally regulated, and starvation induced uptake of ammonium into Arabidopsis roots. Plant Cell 11, 937–947.
  • Glass A.D.M. & Siddiqi M.Y. (1995) Nitrogen absorption by plant roots. In Nitrogen Nutrition in Higher Plants (eds H.S.Srivastava & R.P.Singh), pp. 21–56. Associated Publishing Co., New Delhi, India.
  • Glass A.D.M., Brito D.T., Kaiser B.N., et al. (2001) Nitrogen transport in plants, with an emphasis on the regulation of fluxes to match plant demand. Journal of Plant Nutrition and Soil Science 164, 199–207.
    Direct Link:
  • Glass A.D.M., Britto D.T., Kaiser B.N., et al. (2002) The regulation of nitrate and ammonium transport systems in plants. Journal of Experimental Botany 53, 855–864.
  • Glass A.D.M., Erner Y., Kronzucker H.J., Schjoerring J.K., Siddiqi M.Y. & Wang M.Y. (1997) Ammonium fluxes into plant roots: energetics, kinetics and regulation. Journal of Plant Nutrition and Soil Science 160, 261–268.
    Direct Link:
  • Goyal S.S. & Huffaker R.C. (1984) Nitrogen toxicity in plants. In Nitrogen in Crop Production (ed. R.D.Hauck). ASA, CSSA, SSSA, Madison, WI, USA.
  • Hall T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.
  • Jackson W.A., Chaillon S., Morot-Gaudry J.-F. & Volk R.J. (1993) Endogenous ammonium generation in maize roots and its relationship to other ammonium fluxes. Journal of Experimental Botany 44, 731–739.
  • Kaiser B.N., Rawat S.R., Siddiqi M.Y., Masle J. & Glass A.D.M. (2002) Functional analysis of an Arabidopsis T-DNA ‘knockout’ of the high-affinity NH4+ transporter AtAMT1; 1. Plant Physiology 130, 1263–1275.
  • Kosola K.R. & Bloom A.J. (1994) Methylammonium as a transport analogue for ammonium in tomato (Lycopersicon esculentum L.). Plant Physiology 105, 435–442.
  • Kronzucker H.J., Siddiqi M.Y. & Glass A.D.M. (1995a) Compartmentation and flux characteristics of ammonium in spruce. Planta 196, 691–698.
  • Kronzucker H.J., Siddiqi M.Y. & Glass A.D.M. (1995b) Analysis of 13NH4+ efflux in spruce roots: a test case for phase identification in compartmental analysis. Plant Physiology 109, 481–490.
  • Kronzucker H.J., Siddiqi M.Y. & Glass A.D.M. (1996) Kinetics of NH4+ influx in spruce. Plant Physiology 110, 773–779.
  • Larsson C.-M., Larsson M., Purves J.V. & Clarkson D.T. (1991) Translocation and cycling through roots of recently absorbed nitrogen and sulphur in wheat (Triticum aestivum) during vegetative and generative growth. Physiologia Plantarum 67, 30–36.
  • Lauter F.-R., Ninnemann O., Bucher M., Riesmeier J.W. & Frommer W.B. (1996) Preferential expression of an ammonium transporter and of two putative nitrate transporters in root hairs of tomato. Proceedings of National Academy of Science USA 93, 8139–8144.
  • Lee R.B. & Ayling S.M. (1993) The effect of methionine sulfoximine on the absorption of ammonium by maize and barley roots over short periods. Journal of Experimental Botany 44, 53–63.
  • Lee R.B. & Rudge K.A. (1986) Effects of nitrogen deficiency on the absorption of nitrate and ammonium by barley plants. Annals of Botany 57, 471–486.
  • Lejay L., Tillard P., Lepetit M., Olive F.D., Filleur S., Daniel-Vedele F. & Gojon A. (1999) Molecular and functional regulation of two NO3– uptake systems by N- and C-status of Arabidopsis plants. Plant Journal 18, 509–519.
    Direct Link:
  • Liu Z., Chen Y., Mo R., Hui C., Cheng J.F., Mohandas N. & Huang C.H. (2000) Characterization of human RhCG and mouse Rhcg as novel nonerythroid Rh glycoprotein homologues predominantly expressed in kidney and testis. Journal of Biology Chemistry 275, 25641–25651.
  • Ludewig U., Von Wiren N. & Frommer W.B. (2002) Uniport of NH4+ by the root hair plasma membrane ammonium transporter LeAMT1; 1. Journal of Biology Chemistry 277, 13548–13555.
  • Marini A.-M., Matassi G., Raynal V., Andre B. & Cartron J.-P. (2000) The human rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast. Nature Genetics 26, 341–344.
  • Marini A.-M., Soussi-Boudekou A., Vissers S. & Andre B. (1997) A family of ammonium transporters in Saccharomyces cerevisiae. Molecular and Cellular Biology 17, 4282–4293.
  • Marini A.-M., Vissers S., Urrestarazu A. & Andre B. (1994) Cloning and expression of the MEP1 gene encoding a transporter of ammonium in Saccharomyces cerevisiae. European Molecular Biology Organization Journal 13, 3456–3463.
  • Matt P., Geiger M., Walch-Liu P., Engels C., Krapp A. & Stitt M. (2001) The immediate cause of the diurnal changes of nitrogen metabolism in leaves of nitrate-replete tobacco: a major imbalance between the rate of nitrate reduction and the rates of nitrate uptake and ammonium metabolism during the first part of the light period. Plant, Cell and Environment 24, 177–190.
    Direct Link:
  • Meeks J.C., Wolk C.P., Lockau W., Schillimg N., Jeseph C.M. & Chien W.-S. (1978) Pathways of assimilation of [13]N2 and 13NH4+ by cyanobaceria with and without heterocysts. Journal of Bacteriology 134, 125–130.
  • Morgan M.A. & Jackson W.A. (1988) Inward and outward movements of ammonium in root system: transient responses during recovery from nitrogen deprivation in presence of ammonium. Journal of Experimental Botany 39, 179–191.
  • Nicholas K.B. & Nicholas H.B. Jr (1997) GeneDoc: a tool for editing and annotating multiple sequence alignments. Distributed by the author.
  • Ninnemann O., Jauniaux J.-C. & Frommer W.B. (1994) Identification of a high affinity ammonium transporter from plants. European Molecular Biology Organization Journal 13, 3464–3471.
  • Pearson J.N., Finnemann J. & Schjoerring J.K. (2002) Regulation of high-affinity ammonium transporter (BnAMT1; 2) in the leaves of Brassica napus by nitrogen status. Plant Molecular Biology 49, 483–490.
  • Persson B. & Argos P. (1994) Prediction of transmembrane segments in proteins utilizing multiple sequence alignments. Journal of Molecular Biology 237, 182–192.
  • Rawat S.R., Silim S.N., Kronzucker H.J., Siddiqi M.Y. & Glass A.D.M. (1999) AtAMT1 gene expression and NH4+ uptake in roots of Arabidopsis thaliana: evidence for regulation by root glutamine level. Plant Journal 19, 143–152.
    Direct Link:
  • Salvemini F., Marini A.-M., Riccio A., Patriarca E.J. & Chiurazzi M. (2001) Functional characterization of an ammonium transporter gene from Lotus japonicus. Gene 270, 237–243.
  • Sasakawa H. & Yamamoto Y. (1978) Comparison of the uptake of nitrate and ammonium by rice seedlings, influences of light, temperature, oxygen concentrations, exogenous sucrose, and metabolic inhibitors. Plant Physiology 62, 665–669.
  • Siddiqi M.Y., Glass A.D.M., Ruth T.J. & Fernando M. (1989) Studies of the regulation of nitrate influx by barley seedlings using 13NO3–. Plant Physiology 90, 806–813.
  • Sohlenkamp C., Shelden M., Howitt S. & Udvardi M. (2000) Characterization of Arabidopsis AtAMT2 a novel ammonium transporter in plants. Federation of European Biochemical Society Letters 467, 273–278.
  • Solorzano L. (1969) Determination of ammonium in natural waters by the phenyl hypochlorite method. Limnology and Oceanography 14, 799–801.
  • Sonnhammer E.L.L., Von Heijne G. & Krogh A. (1998) A hidden Markov model for predicting transmembrane helices in protein sequences. In Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology (eds J.Glasgow, T.Littlejohn, F.M.R.Lathrop, D.Santoff & C.Sensen ), pp. 175–182. AAAI Press, Menlo Park, CA, USA.
  • Suzuki I., Sugiyama I. & Omata I. (1993) Primary structure and transcriptional regulation of gene for nitrite reductase from the cyanobacterium Synechococcus PCC 7942. Plant and Cell Physiology 34, 13311–11320.
  • Thomas T.E. & Harrison P.J. (1985) Effect of nitrogen supply on nitrogen uptake, accumulation and assimilation in Porphyra perforata (Rhodophyta). Marine Biology 85, 269–278.
  • Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. & Higgins D.G. (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.
  • Thompson J.D., Higgins D.G. & Gibson T.J. (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673–4680.
  • Ullrich W.R., Larsson M., Larsson C.-M., Lesch S. & Novacky A. (1984) Ammonium uptake in Lemna gibba G1, related membrane potential changes and inhibition anion uptake. Physiologia Plantarum 61, 369–376.
    Direct Link:
  • Vaalburg W., Kamphuis J.A.A., Beerling-van der Molen H.B., Reiffers S., Rijskamp A. & Woldring M.G. (1975) An improved method for the cyclotron production of 13N-labelled ammonia. International Journal of Applied Radiation and Isotopes 26, 316–318.
  • Wang M.Y., Glass A.D.M., Shaff J.E. & Kochian L.V. (1994) Ammonium Uptake by rice roots III. Electrophysiology. Plant Physiology 104, 899–906.
  • Wang M.Y., Siddiqi M.Y., Ruth T.J. & Glass A.D.M. (1993a) Ammonium uptake by rice roots I. Fluxes and subcellular distribution of 13NH4+. Plant Physiology 103, 1249–1258.
  • Wang M.Y., Siddiqi M.Y., Ruth T.J. & Glass A.D.M. (1993b) Ammonium uptake by rice roots II. Kinetics of 13NH4+ across plasmalemma. Plant Physiology 103, 1259–1267.
  • Wiame J.M., Grenson M. & Arst H.N. (1985) Nitrogen catabolite repression in yeasts and filamentous fungi. Advances in Microbial Physiology 26, 1–88.
  • Von Wiren N., Lauter F.-R., Ninnemann O., Gillissen B., Walch-Liu P., Engels C., Jost W. & Frommer W.B. (2000) Differential regulation of three functional ammonium transporter genes by nitrogen in root hairs and by light in leaves of tomato. Plant Journal 21, 167–175.
    Direct Link:
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