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Keywords:

  • Phaseolus vulgaris;
  • Pisum sativum;
  • CO2 assimilation rate;
  • internal conductance;
  • Rubisco degradation;
  • stomatal conductance;
  • vapour pressure deficit

ABSTRACT

  1. Top of page
  2. ABSTRACT
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. ACKNOWLEDGMENTS
  8. REFERENCES

Leaf surface wetness that occurs frequently in natural environments has a significant impact on leaf photosynthesis. However, the physiological mechanisms for the photosynthetic responses to wetness are not well understood. The responses of leaf CO2 assimilation rate (A) to 72 h of artificial mist of a wettable (bean; Phaseolus vulgaris) and a non-wettable species (pea; Pisum sativum) were compared. Stomatal and non-stomatal limitations to A were investigated. A 28% inhibition of A was observed in the bean leaves as a result of a 16% decrease in stomatal conductance and a 55% reduction in the amount of Rubisco. The decrease of Rubisco was mainly due to its partial degradation. In contrast to the bean leaves, a 22% stimulation of A was obtained in the 72 h mist-treated pea leaves. Mist treatment increased stomatal conductance by 12.5% and had no effect on the amount of Rubisco. These results indicated that a positive photosynthetic response to wetness occurred only in non-wettable species and is due to the change in stomatal regulation.


INTRODUCTION

  1. Top of page
  2. ABSTRACT
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. ACKNOWLEDGMENTS
  8. REFERENCES

In a natural environment, the leaf surfaces of plants frequently are wetted by rain, fog and dew. Surface wetness induces different changes in leaf photosynthesis among species, because leaf surface wettability varied greatly, that is, from being covered almost completely by water to being water repellent (Smith & McClean 1989; Brewer, Smith & Vogelmann 1991; Brewer & Smith 1997). In alpine and sub-alpine plants, natural dew depressed A by 77% in species having wettable leaves, whereas A was stimulated by 14% in species having non-wettable leaves (Smith & McClean 1989).

The different responses of A to wetting have been attributed to differences in the temporal impact of surface water on the stomata. Water droplets on the leaves may reduce the water vapour deficit (Smith & McClean 1989). Wetness may thus stimulate stomatal conductance and thus A in ‘non-wettable’ species. The reduction of A by wetness in the ‘wettable’ species may be partly due to the physical blockage of stomata by the surface water (Brewer & Smith 1995). In these cases, photosynthetic responses will be reversible, i.e. A will fully recover when water droplets are removed from the leaf surface.

However, the reduction of A by wetness is irreversible when leaves of a ‘wettable’ species (bean; Phaseolus vulgaris) are subjected to long-term mist exposure. A reduction in A was observed in 24 h mist-exposed bean leaves after removing water droplets (Ishibashi & Terashima 1995; Ishibashi, Usuda, & Terashima 1996). However, little is known about the mechanism of the irreversible response or whether it differs among species.

The ‘irreversible’ response of A to surface wetness may be caused by some alterations in the two main limiting processes: (1) CO2 diffusion processes; and (2) CO2 fixation processes mediated by Rubisco. Surface wetness should affect the signals controlling stomatal regulation, such as water loss rate (Mott & Parkhurst 1991; Monteith 1995) and leaf water potential (Comstock & Mencuccini 1998). Surface wetness may affect leaf internal CO2 diffusion through the alteration of leaf morphology. A morphological change by wetness (Kimura 1987) may involve an alteration in mesophyll anatomy such as chloroplast distribution and cell wall thickness, which determines internal conductance (Evans et al. 1994; Kogami et al. 2001).

The objective of this study is to clarify the mechanisms for the response of A to surface wetness for species with different wettability. We focused on the ‘irreversible’ effects of wetting on photosynthetic machinery. A 72 h mist pretreatment was conducted to compare the A between bean (Phaseolus vulgaris) and pea (Pisum sativum). Bean leaves have a large and flat surface that is wetted with ease (wettable), whereas pea leaves have a smooth and waxy surface that is difficult to wet (non-wettable, Tukey 1970). Gas exchange parameters and the amount of Rubisco in the leaves were analysed as possible factors underlying the photosynthetic response. We obtained different responses of A between bean and pea, which was governed by changes in stomatal limitation and the amount of Rubisco.

MATERIALS AND METHODS

  1. Top of page
  2. ABSTRACT
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. ACKNOWLEDGMENTS
  8. REFERENCES

Plant materials

Seeds of bean (Phaseolus vulgaris L. cv. Kurosando-saito) and pea (Pisum sativum L. cv. Denko) were obtained commercially (Takii Seeds, Kyoto, Japan). Each of the seedlings was transplanted to vermiculite in a 600-cm3 pot and grown in a growth chamber (LPH-1000-NCR; Nippon Medical and Chemical Instruments, Osaka, Japan). The chamber was kept at 25 °C, 80% relative humidity, 300 µmol m−2 s−1 PPFD, and 12 h photoperiod. The light was provided by four high-intensity discharge lamps (DR400/T-L; Toshiba, Tokyo, Japan). Plants were fertilized daily with a half-strength Hoagland solution (Hewitt & Smith 1975).

For the wetness pretreatment, the mist of deionized water (pH = 5.1) was applied uniformly to the plants from four spray nozzles at a rate of 470 cm3 h−1. The soil surface of the pots was covered with a plastic film to prevent the soil from being wetted by the mist. At the start of the mist pretreatment the leaf age was 10 d for the primary leaves of bean and 7 d for the pea leaves.

Leaf surface properties

The apparent contact angle of water droplet (θ) was measured with a contact angle-meter (model G-1; Erma Inc., Tokyo, Japan) for a 5-mm3 water droplet placed onto the leaf surface. A lower value of θ indicates a less spherical water droplet and thus a more-wettable surface (Brewer et al. 1991). To measure the amount of water on the leaves, 50 cm3 of water was sprayed on a pre-weighed leaf with a cone nebulizer, and then the leaf was immediately re-weighed.

Water absorption during the mist pretreatment may affect leaf water potential and thus stomatal regulation. Therefore, we checked whether bean and pea leaves were able to absorb water directly from the leaf surface, using fluorescence microscopy. The upper surface of the intact leaves was sprayed with a 0.1% (w/v) aqueous solution of fluorescent brightener-28 (Sigma-Aldrich, St Louis, MO, USA), which binds to the polysaccharides of the cell walls and emits blue fluorescence under a wavelength of 350 nm (Munné-Bosch, Nogués & Alegre et al. 1999). After 30 min, these leaves were washed in distilled water, and then their transverse sections were stained in distilled water in order to observe them under a fluorescence microscope (BX51; Olympus, Tokyo, Japan) at 350 nm.

Gas exchange measurements

Gas exchange measurements were made with a system described by Hanba, Kogami & Terashima (2002) for intact leaves. Measurements were repeated twice after a 10-min acclimation to 740 µmol m−2 s−1 PPFD for each of the leaves. The light source was a quartz-halogen lamp (350 W) with a reflector for far-red light. In the course of the measurements, leaf temperature and ambient CO2 partial pressure was kept at 24.0 ± 1.0 °C and 35 ± 0.4 Pa, respectively.

Before the mist pretreatment, the temporal effects of surface water on CO2 assimilation rate (A) were detected by measuring the time-course of A after spraying distilled water on the upper surface of the leaves. In the bean leaves, water droplets covered their surfaces almost completely. The water droplets on the leaves were lost by evaporation after 10 min. For the pea leaves, the water droplets covered only a small part of the leaves.

For the measurements of the leaves from the 24–72 h mist pretreatment, water droplets on the leaves were carefully removed using filter papers, and then the gas-exchange was measured after 10-min air-drying in laboratory conditions. The stomatal response to vapour pressure deficit (VPD) was analysed by changing the vapour pressure of the air entering the assimilation chamber.

Detection of Rubisco and its degradation products

Three leaf discs (1.8 cm2 × 3) were obtained from the central part of the lamina, and immediately homogenized at 4 °C in a 50-mm HEPES buffer (pH 7.5) containing 0.2% triton X-100, 0.7% 2-mercaptoethanol, 2 mm monoiodoacetic acid, 25% glycerol, 6% lithium dodecyl sulfate (LDS), and 1% polyvinyl polypyrrolidone (PVP). No protease inhibitor was added to the buffer. These homogenates were immediately centrifuged, and their supernatants (0.2 cm3) were boiled for 5 min with the Laemmli buffer (Laemmli 1970). The proteins in the supernatants were separated using 14% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The SDS-PAGE gels were stained with a Coomassie Brilliant Blue G-250 (Bio-safe Coomassie; Bio-Rad, Hercules, CA, USA). The amount of Rubisco subunits was determined spectrophotometrically by scanning the gel with a scanner (GT7600U; Epson, Tokyo, Japan), analysed using software (Densitograph; ATTO, Tokyo, Japan).

After the electrophoretic separation by SDS-PAGE was completed, the proteins were transferred onto polyvinylidene fluoride membranes (Immune-blot PVDF membrane; Bio-Rad) using a semi-dry system (NA-1515; Nippon-Eido, Tokyo, Japan). Rubisco was detected with an antibody against Rubisco (1/3000 dilution) and visualized using an alkaline phosphatase kit (AP conjugate substrate kit; Bio-Rad). Band analysis was performed as described in the previous section.

Leaf morphology and biochemistry

Stomatal density and size of stomata were measured to obtain the effect of the mist pretreatment on stomata of bean and pea. A mixture of a silicone rubber monomer (KE-10; Shinetsu Chemical, Tokyo, Japan) and a catalyst (CAT-RA; Shinetsu Chemical) was poured on the upper and lower side of the leaves (n = 5). The impression was cleaned using 99% ethanol, and then a secondary replica was taken by applying nail varnish. Ten stomata were randomly taken to determine the size of stomata for each of the replicas, analysed with a light micrograph using software (NIH Image; National Institute of Health, Bethesda, MD, USA).

Chlorophyll was extracted in 4 cm3 dimethylformamide from a leaf disc (1.8 cm2), obtained from the central part of the lamina. Chlorophyll concentration was determined spectrophotometrically from the absorbance at wavelengths of 663.8 and 646.8 nm (U-1800; Hitachi, Tokyo, Japan) following Porra, Thompson & Kriedemann (1989).

For the analysis of soluble protein, a leaf disc (1.8 cm2) was obtained from the central part of the lamina, and immediately homogenized at 4 °C in a 50-mm HEPES buffer (pH 7.5) containing 0.7% polyethylene glycol 2000 (w/v), 0.2% PVP, and 1 mm phenylmethylsulfonyl fluoride (PMSF). These homogenates were immediately centrifuged, and the amount of protein was determined for their supernatants using the Bradford method.

Leaf discs (1.8 cm2) were cut from the central part of the lamina, kept in the dark for 15 min to measure initial chlorophyll fluorescence (Fv/Fm) using a portable fluorometer (Plant Efficiency Analyser; Hansatech, Kings Lynn, Norfolk, UK).

We checked the effect of the mist pretreatment on mesophyll anatomy and internal conductance for CO2 diffusion (gi) in the bean leaves. These effects were expected only for the ‘wettable’ species, because a previous study for barley cultivars showed a significant alteration in leaf morphology in the ‘wettable’ mutant but much less alteration in the ‘non-wettable’ wild type (Takehana et al. unpublished work).

Leaf mesophyll anatomy was analysed using light microscopy, as described in Hanba et al. (2002). Transverse slices 1 mm × 3 mm were cut from the central part of the lamina, fixed in 2.5% glutaraldehyde and 2% osmium tetroxide, then embedded in Spurr's resin (Spurr 1969). Transverse sections, 700 nm thick, were stained in a 1% toluidine blue solution, and light micrographs were taken with a digital camera (DP11; Olympus, Tokyo, Japan). Anatomical characteristics were determined from these 150 µm-wide, digitized images using software (NIH Image).

Internal conductance to CO2 diffusion was estimated by concurrent measurements of gas exchange and carbon isotope ratio as described by Hanba et al. (2002). The conditions for the gas exchange measurements were as described in the previous section. Carbon isotope ratios were measured with a mass spectrometer (Finnigan MAT 252, Bremen, Germany).

Statistics

Effects of treatment were tested with a t-test using StatView (SAS Institute, Cary, NC, USA) at a probability level of P < 0.05.

RESULTS

  1. Top of page
  2. ABSTRACT
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. ACKNOWLEDGMENTS
  8. REFERENCES

Leaf surface properties related to the surface wettability were characterized for bean and pea (Table 1). The bean leaves had a much lower apparent contact angle of a water droplet (θ) compared with those of the pea (Table 1). In addition, the bean leaves retained 1.7-fold more water than pea leaves (Table 1). These measurements showed that the bean leaves had more wettable surfaces in comparison with the pea leaves.

Table 1.  Leaf surface properties of the upper and lower sides of the leaves of bean (Phaseolus vulgaris) and pea (Pisum sativum). Leaf age was 10 d in bean and 7 d in pea. A high contact angle of water droplets indicates a more water repellent surface
CharacteristicSurfaceBean (wettable)Pea (non-wettable)
  1. Data are mean (SD) for n = 5.

Leaf area (cm2) 41.9 (5.5)  2.6 (0.6)
Amount of retained water (mg cm−2)Upper20.2 (3.9) 12.0 (4.7)
Lower21.0 (1.8) 11.6 (5.5)
Contact angle of water droplet (°)Upper72.6 (8.3)117.4 (11.1)
Lower66.3 (9.3)129.4 (10.0)
Leaf hairsUpperPresentAbsent
LowerPresentAbsent

The bean leaves that were sprayed with an aqueous solution of fluorescence showed blue fluorescence on epidermal and mesophyll cell walls, whereas no blue fluorescence was observed in the pea leaves (Fig. 1). This result indicates that surface water entered the mesophyll cell through the epidermis in the bean leaves but not in the pea leaves.

image

Figure 1. Leaf transverse sections of bean (Phaseolus vulgaris) and pea (Pisum sativum) observed with a fluorescent microscope at 350 nm. Upper surfaces of the leaves were sprayed with distilled water (a, b) or aqueous solution of fluorescent brightener-28 (c, d). Fluorescent brightener was used as a tracer of apoplastic water. Strong blue fluorescence was observed only in the bean section sprayed with a fluorescent brightener.

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The temporal effects of surface water on leaf CO2 assimilation rate (A) was compared between bean and pea (Fig. 2). A remarkable reduction of A was obtained in the wet leaves of bean, and then A recovered rapidly with the evaporation of the surface water. Although the surface water on the bean leaves was lost almost completely after 10 min, the reduction of A was still observed. In contrast, no reduction of A was obtained in the wet leaves of pea.

image

Figure 2. Temporal effects of surface water on leaf CO2 assimilation rate (A) of bean [Phaseolus vulgaris; (a)] and pea [Pisum sativum, (b)]. After the measurements of A for the dry leaves, their upper surfaces were misted with distilled water. These wet leaves were held horizontally under high irradiance (740 µmol m−2 s−1 PPFD) for 5 min, and then the time-course of A was analysed. The effect of CO2 absorption by the surface water was so small that it was negligible in this experiment. Changes in A in the wet leaves are shown as values relative to the A in dry leaves. Values were mean ± SD (n = 3).

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The effect of 72 h mist pretreatment on leaf CO2 assimilation rate (A) and stomatal conductance (gs) was compared between bean and pea (Fig. 3). In control leaves of bean and pea, both A and gs were constant during the experiment (data not shown). A significant inhibition of A was obtained in bean, contrasting with the stimulation of A in pea (Fig. 3a). A 72 h pretreatment inhibited A by 28% in bean, whereas it enhanced A by 22% in pea. The response of gs in bean was different from that in pea (Fig. 3b). The 72 h pretreatment decreased gs by 16% in bean, whereas gs was stimulated by 12.5% in pea (Fig. 3b).

image

Figure 3. Leaf CO2 assimilation rate (a) and CO2 conductance (b) of bean (Phaseolus vulgaris) and pea (Pisum sativum) measured at a 740 µmol m−2 s−1 PPFD in the course of mist pretreatment. Vapour pressure deficit (VPD) was kept at 700 ± 53 Pa during the measurements. Measurements were recorded after removing water droplets from the leaf surface. Values indicate means ± SD (n = 6–16).

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The responses of stomatal conductance (gs) to VPD were compared between no-mist and 72 h mist-treated leaves to investigate the effect of mist pretreatment to stomatal regulation (Fig. 4). The mist-treated bean leaves showed lower gs in comparison with the control leaves from 500 to 1700 Pa VPD. In contrast, the mist-treated pea leaves had higher gs than the control leaves. The sensitivity of stomata to VPD was similar between species in the control leaves, whereas in the mist-treated leaves, pea leaves showed high sensitivity to VPD in comparison with the bean leaves.

image

Figure 4. Changes in the response of stomatal conductance to vapour pressure deficit of bean (Phaseolus vulgaris) and pea (Pisum sativum) by mist pretreatment. Solid lines show regression lines for each of the pretreatments. Values were measured after removing water droplets from the leaf surface of three leaves for each of the pretreatments. r2 = 0.92, 0.92, 0.93 and 0.93 for the no-mist pea, no-mist bean, 72 h-mist pea and 72 h-mist bean leaves, respectively.

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The amount of proteins of the leaf extracts was detected by SDS-PAGE using a Coomassie-blue stain, and the degradation products of Rubisco in the leaf extract was detected using a western blot (Fig. 5). In the Coomassie stain, a most abundant protein of 53 kDa was attributed to the Rubisco large subunit (LSU). A 15-kDa band was attributed to the Rubisco small subunit (SSU). Mist pretreatment reduced the amount of LSU and SSU in bean, whereas no such reductions were observed in pea. Western blot data showed that with the major band of 53 kDa, fragments of 42, 31 and 23 kDa were recognized in the extracts of both of the species. They were likely to be the degradation products of Rubisco. Mist pretreatment increased the intensities of these fragments in the bean leaves, whereas the pretreatment effect was less in the pea plants.

image

Figure 5. SDS-PAGE fractionation of the leaf extracts of bean (Phaseolus vulgaris) and pea (Pisum sativum) before and after the 72 h mist pretreatment. These extracts were stained with a Coomassie Brilliant Blue (left) or subjected to a western blot analysis using an antibody against Rubisco (right).

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The effect of mist pretreatment on the amount of Rubisco was quantified using a Coomassie stain of the SDS-PAGE gel (Fig. 6). At the start of the pretreatment, the amount of LSU was 3.3 ± 0.2 and 4.2 ± 0.3 g m−2 in the bean and the pea leaves, respectively (mean ± SD, n = 5). The amount of LSU was reduced by 55% after the 72 h mist pretreatment in the bean leaves, but no reduction was obtained in the pea leaves. Similarly, the amount of SSU was decreased by 34% in the 72 h mist-treated bean leaves, whereas no change was obtained for the pea leaves.

image

Figure 6. Amounts of Rubisco large subunit (LSU) and small subunit (SSU) during the mist pretreatment. Rubisco was detected by SDS-PAGE of the leaf extracts, stained with a Coomassie Brilliant Blue. Relative Rubisco content (Rubisco amount with mist/Rubisco amount without mist) is shown. The amount of LSU and SSU were estimated from the density of the 53 and 15 kDa bands, respectively. The amount of Rubisco LSU at the start of the pretreatment was 3.3 (± 0.2) g m−2 and 4.2 (± 0.3) g m−2 for the bean (Phaseolus vulgaris) and the pea (Pisum sativum) leaves, respectively (n = 5). Values are means ± SD (n = 5).

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Rubisco degradation was analysed using Western blot (Fig. 7). The breakdown products of 42 and 23 kDa were increased after 72 h mist pretreatment in bean, whereas no effect of pretreatment was observed in pea. The amount of these breakdown products was higher in bean than those in pea.

image

Figure 7. Effect of 72 h mist pretreatment on the degradation products (42, 31 and 23 kDa) from the Rubisco large subunit (LSU) of bean (Phaseolus vulgaris) and pea (Pisum sativum). These proteins were detected by a western blot analysis using an antibody against Rubisco. Data are shown as values relative to the amount of LSU. Values are means ± SD (n = 5–10).

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The effect of 72 h mist pretreatment on stomatal distribution was examined for bean and pea. Leaf morphology, chlorophyll concentration and Fv/Fm were little affected for both species (Table 2). On the other hand, stomatal density was increased in pea but not in bean. Increases in the size of stomata and in the amount of soluble protein were obtained in the bean only.

Table 2.  Effects of 72 h mist pretreatment on leaf characteristics of bean (Phaseolus vulgaris) and pea (Pisum sativum)
ParameterBeanPea
Control72 h mistControl72 h mist
  1. Data are mean (SD) for n = 5. Means followed by different letters are statistically different, tested by t-test (P < 0.05).

Stomatal density
 Upper surface (no. mm−2)  37 (5)  32 (6)  69 (5)  66 (9)
 Lower surface (no. mm−2) 179 (9) 161 (23)  96 (10) a 114 (8) b
Stomata size
 Upper surface (µm)25.6 (2.0) a32.5 (2.1) b11.2 (0.4)11.8 (0.7)
 Lower surface (µm)24.5 (2.0) a28.8 (1.8) b10.8 (0.2)10.8 (0.4)
Leaf dry mass per area (g m−2)19.8 (0.6)18.3 (1.7)24.1 (2.9)20.3 (3.2)
Chlorophyll a+b (µmol m−2) 359 (53) 324 (49) 469 (23) 469 (31)
Soluble protein (g m−2)3.10 (0.18) a3.92 (0.70) b4.53 (0.97)4.00 (0.20)
Fv/Fm0.80 (0.03)0.83 (0.01)0.80 (0.01)0.82 (0.002)

Leaf internal conductance (gi) and related mesophyll anatomy were estimated in the bean plants only to examine the mesophyll limitation to A(Table 3). The value of gi was not affected by the mist pretreatment in the bean leaves. Mesophyll thickness and surface area of mesophyll cells exposed to intercellular airspaces (Smes) were unaffected by the pretreatment, whereas the surface area of chloroplasts facing to intercellular airspaces (Sc) declined slightly.

Table 3.  The effects of 72 h mist pretreatment on the leaf mesophyll anatomy and internal CO2 conductance in bean (Phaseolus vulgaris). Smes or Sc indicates surface area of mesophyll or chloroplasts exposed to intercellular airspaces. No analysis was done for the pea leaves, because mist treatment causes no morphological alteration in pea (data not shown)
ParameterControl72 h mist
  1. Data indicate mean ± SD (n = 5). Difference of means was tested as described in Table 1.

Mesophyll thickness (µm)139 (11)138 (21)
Mesophyll porosity (m3 m−3)0.38 (0.04) a0.45 (0.02) b
Smes(m2 m−2)18 (2)16 (2)
Sc(m2 m−2)14 (1) a11 (1) b
Internal conductance (mol m−2 s−1)0.151 (0.009)0.151 (0.021)

DISCUSSION

  1. Top of page
  2. ABSTRACT
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. ACKNOWLEDGMENTS
  8. REFERENCES

Our finding that exposing bean leaves to 5 min of mist resulted in a significant and temporal reduction of the CO2 assimilation rate (A, Fig. 2) suggest that stomatal blockage by the film of surface water strongly inhibits CO2 diffusion in ‘wettable’ species. This result supports previous reports that, at least for ‘wettable’ species, a temporal response of stomata to water droplets on the leaves had a significant impact on photosynthesis (Smith & McClean 1989; Brewer & Smith 1995). The reduction in A was recovered by 95% when water droplets on the leaves were evaporated (Fig. 2). However, when the mist exposure was prolonged to 72 h, a 28% reduction of A was observed after removing water droplets from the leaves for the ‘wettable’ species (Fig. 3). In addition, for the ‘non-wettable’ species, the 72 h mist pretreatment increased A significantly, although no effect of mist was observed for the leaves exposed to 5 min of mist (Fig. 2). These indicate that mist has ‘irreversible’ effects on photosynthesis for both of the ‘wettable’ and ‘non-wettable’ species, if their leaves are exposed to prolonged mist.

In pea, 72 h mist pretreatment resulted in an increase in stomatal conductance (gs; Fig. 3b) and no change in the amount of Rubisco (Fig. 6). This suggests that the increase of A in pea (Fig. 3a) is due to an enhancement of CO2 diffusion through stomata. In a natural environment, the enhancement of CO2 diffusion in mist-treated leaves was attributed to a decrease in the VPD caused by the surface water (Munné-Bosch et al. 1999). However, gs in the mist-treated pea was constantly higher than that in the control leaves when VPD was changed from 500 to 1500 Pa (Fig. 4). This indicates that 72 h mist pretreatment changes the stomatal response to VPD. A previous study showed that gs was significantly higher for the rice plants pretreated to high humidity than those to low humidity when measured below 2.0 kPa of VPD (Kawamitsu, Yoda & Agata 1993). Similarly, the exposure of cuttings of Corylus maxima to fog increased stomatal conductance under similar VPD (Fordham et al. 2001). Our result is consistent with these previous findings.

This increase in gs by the mist pretreatment may be partly induced by an increase in stomatal density in the mist-treated pea (Table 2). On the other hand, water potential of epidermal cells, which was considered to be a cue for stomatal regulation (Monteith 1995; Buckley & Mott 2002), may not cause the increase in gs because of the absence of water absorption by the leaf surface in the mist-treated pea (Fig. 1).

Mist pretreatment of bean leaves resulted in a reduction in stomatal conductance (Fig. 3b) but no change in internal conductance (Table 3). These results suggest that the inhibition of A by the mist pretreatment (Fig. 3a) is at least partly caused by a limitation of diffusion through the stomata. Water absorption from the leaf surface (Fig. 1) might increase water potential more in the epidermal cells than in the guard cells, causing stomatal closure of the mist-treated bean leaves (Fig. 4). Another possibility is that leaching of the ions from the wetted leaves (Tukey 1970) induces a relative decrease in the osmotic potential of the epidermal cells compared with guard cells, resulting in stomatal closure in the wettable species. The fact that mist pretreatment had no effect on internal conductance may be due to the small change in mesophyll anatomy (Table 3).

Perhaps a more important cause of the reduction in A in bean is a decrease in the level of Rubisco, because the amount of both LSU and SSU were reduced remarkably in this species (Fig. 6). The decrease in LSU is consistent with the finding of Ishibashi et al. (1996) that 24 h mist exposure reduced the amount of Rubisco by 45% in bean leaves. The reduction in the amount of Rubisco may be due to an alteration of the balance of Rubisco synthesis and degradation, because Rubisco normally undergoes continuous degradation in mature leaves (Esquivel, Ferreira & Teixeira 1998). The fragments of 42, 31 and 23 kDa in the present study (Fig. 5) are most likely the degradation product of Rubisco LSU. A 36 to 37 kDa fragment was detected as a degradation product of LSU in rice ( Mae et al. 1983), barley (Desimone, Henke & Wagner 1996) and wheat (Ishida et al. 1997), under oxidative stress. Similarly, proteins of 48, 34 and 24 kDa were detected by a Rubisco antibody in leaf extracts from pea plants, which were probably the degradation products of LSU (Romero-Puertas et al. 2002). Therefore, the increased intensities of the 42 and 23 kDa bands (Fig. 7) indicate accumulations of the degradation products. The reduction in LSU and the increase in degradation products of Rubisco in bean indicate that Rubisco turnover is inhibited by mist pretreatment.

How is Rubisco degraded in mist-treated bean leaves? One possibility is that environmental stresses that limit CO2 fixation cause Rubisco fragmentation (Desimone et al. 1996; Ishida et al. 1997), because energy is channelled from CO2 fixation to the production of active oxygen species resulting in photo-inhibition (Moran et al. 1994; Tambussi et al. 2000). In fact, drought stress, which limits CO2 fixation, reduced the amount of Rubisco in the leaves of tobacco (Gunasekera & Berkowitz 1993) and rice (Vu et al. 1998). However, the Rubisco degradation that we observed was probably not caused by photo-inhibition because mist pretreatment did not reduce Fv/Fm (Table 2). A second possibility is that the rapid reduction in CO2 assimilation rate by surface wetness (Figs 2 & 3a) triggers a signal that accelerates senescence. This, in turn, could lead to Rubisco degradation. A decline in photosynthetic rate below a certain threshold triggered the senescence programme (Gan & Amasino 1997). Surface wetness, like other environmental stresses such as drought, extreme temperature, shading and nutrient deficiency, may act as an environmental cue that initiates senescence in ‘wettable’ species.

In conclusion, mist pretreatment had significant ‘irreversible’ effects on photosynthesis for both ‘wettable’ and ‘non-wettable’ species: 72 h mist pretreatment caused a 28% decrease in A in bean, whereas it caused a 22% increase in A in pea. This difference may be due to a difference in the effect of wetness on stomatal conductance (gs) and the amount of Rubisco in these two species. The decrease of A by wetness in bean is due to the decreases in both gs and the amount of Rubisco. On the other hand, the increase in A by wetness in pea is due to the increase in gs only.

ACKNOWLEDGMENTS

  1. Top of page
  2. ABSTRACT
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. ACKNOWLEDGMENTS
  8. REFERENCES

This study was supported by a grant from the Ministry of Education, Science, Sports and Culture (no. 12740423) and the Ohara foundation from the Research Institute for Bioresources, Okayama University. The authors thank Dr Y. Yamamoto (Okayama University) for generously donating the antibodies used in this study, and for critical comments on the manuscript. We thank Dr T. Kobayashi (Kagawa University) for constructive comments on the manuscript. The isotope measurements were done at Center for Ecological Research, Kyoto University.

REFERENCES

  1. Top of page
  2. ABSTRACT
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. ACKNOWLEDGMENTS
  8. REFERENCES
  • Brewer C.A. & Smith W.K. (1995) leaf surface wetness and gas exchange in the pond lily Nuphar polysepalum (Nymphaeaceae). American Journal of Botany 0, 12711277.
  • Brewer C.A. & Smith W.K. (1997) Patterns of leaf surface wetness for montane and subalpine plants. Plant, Cell and Environment 20, 111.
  • Brewer C.A., Smith W.K. & Vogelmann T.C. (1991) Functional interaction between leaf trichomes, leaf wettaability and the optical properties of water droplets. Plant, Cell and Environment 14, 955962.
  • Buckley T.N. & Mott K.A. (2002) Dynamics of stomatal water relations during the humidity response: implications of two hypothetical mechanisms. Plant, Cell and Environment 25, 407419.
  • Comstock J. & Mencuccini M. (1998) Control of stomatal conductance by leaf water potential in Hymenoclea salsola (T. & G.), a desert subshrub. Plant, Cell and Environment 21, 10291038.
  • Desimone M., Henke A. & Wagner E. (1996) Oxidative stress induces partial degradation of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase in isolated chloroplasts of barley. Plant Physiology 11, 789796.
  • Esquivel M., Ferreira R. & Teixeira A. (1998) Protein degradation in C3 and C4 plants with particular reference to ribulose bisphosphate carboxylase and glycolate oxidase. Journal of Experimental Botany 49, 807816.
  • Evans J.R., Von Caemmerer S., Setchell B.A. & Hudson G.S. (1994) The relationship between CO2 transfer conductance and leaf anatomy in transgenic tobacco with reduced content of Rubisco. Australian Journal of Plant Physiology 21, 475495.
  • Fordham M.C., Harrison-Murray R.S., Knight L.J. & Evered C.E. (2001) Effects of leaf wetting and high humidity on stomatol function in leafy cuttings and intact plants of Corylus maxima. Physiologia Plantarum 113, 233240.
  • Gan S. & Amasino R.M. (1997) Making sense of senescence (molecular genetic regulation and manipulation of leaf senescence). Plant Physiology 113, 313319.
  • Gunasekera D. & Berkowitz G.A. (1993) Use of transgenic plants with ribulose-1,5-bisphosphate carboxylase/oxygenase antisense dna to evaluate the rate limitation of photosynthesis under water stress. Plant Physiology 103, 629635.
  • Hanba Y.T., Kogami H. & Terashima I. (2002) The effect of growth irradiance on leaf anatomy and photosynthesis in Acer species differing in light adaptation. Plant, Cell and Environment 25, 10211030.
  • Hewitt E.J. & Smith T.A. (1975) Plant Mineral Nutrition (Volume trans.). English University Press, London, UK.
  • Ishibashi M. & Terashima I. (1995) Effects of continuous leaf wetness on photosynthesis: adverse aspects of rainfall. Plant, Cell and Environment 18, 431438.
  • Ishibashi M., Usuda H. & Terashima I. (1996) The loss of ribulose-1,5-bisphosphate carboxylase/oxygenase caused by 24-hour rain treatment fully explains the decrease in the photosynthetic rate in bean leaves. Plant Physiology 111, 635640.
  • Ishida H., Nishimori Y., Sugisawa M., Makino A. & Mae T. (1997) The large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is fragmented into 37-kDa and 16-kDa polypeptides by active oxygen in the lysates of chloroplasts from primary leaves of wheat. Plant Cell Physiology 38, 471479.
  • Kawamitsu Y., Yoda S. & Agata W. (1993) Humidity pretreatment affects the responses of stomata and CO2 assimilation to vapour pressure difference in C3 and C4 plants. Plant Cell Physiology 34, 113119.
  • Kimura K. (1987) Studies on plant response to rainfall (IX) Effect of the duration and intensity of rainfall on the growth of the bean plant. Journal of Agricultural Meteorology 42, 319327 (in Japanese).
  • Kogami H., Hanba Y.T., Kibe T., Terashima I. & Masuzawa T. (2001) CO2 transfer conductance, leaf structure and carbon isotope discrimination of Polygonum cuspidatumn leaves from low and high altitude. Plant, Cell and Environment 24, 529538.
  • Laemmli U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680685.
  • Mae T., Makino A. & Ohira K. (1983) Changes in the amounts of ribulose bisphosphate carboxylase synthesized and degraded during the life span of rice leaf (Oryza sativa L.). Plant Cell Physiology 24, 10791086.
  • Monteith J.L. (1995) A reinterpretation of stomatal responses to humidity. Plant, Cell and Environment 18, 357364.
  • Moran J., Becana M., Iturbe-Ormaetxe I., Frechilla S., Klucas R. & Aparicio-Trejo P. (1994) Drought induces oxidative stress in pea plants. Planta 194, 346352.
  • Mott K.A. & Parkhurst D.F. (1991) Stomatal responses to humidity in air and helox. Plant, Cell and Environment 14, 509515.
  • Munné-Bosch S., Nogués S. & Alegre L. (1999) Diurnal variations of photosynthesis and dew absorption by leaves in two evergreen shrubs growing in Mediterranean field conditions. New Phytologist 144, 109119.
  • Porra R.J., Thompson W.A. & Kriedemann P.E. (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta 975, 384394.
  • Romero-Puertas M.C., Palma J.M., Gómez , M., Río , L.A.D. & Sandalio L.M. (2002) Cadmium causes the oxidative modification of proteins in pea plants. Plant, Cell and Environment 25, 677686.
  • Smith W.K. & McClean T.M. (1989) Adaptive rrelationship between leaf water repellency, stomatal distribution, and gas exchange. American Journal of Botany 76, 465469.
  • Spurr A.R. (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26, 3143.
  • Tambussi E.A., Bartoli C.G., Beltrano J., Guiamet J.J. & Araus J.L. (2000) Oxidative damage to thylakoid proteins in water-stressed leaves of wheat (Triticum aestivum). Physiologia Plantarum 108, 398.
  • Tukey J.H.B. (1970) The leaching of substances from plants. Annual Review of Plant Physiology and Plant Molecular Biology 21, 305324.
  • Vu J.C.V., Baker J.T., Pennanen A.H., Allen L.H. Jr, Bowes G. & Boote K.J. (1998) Elevated CO2 and water deficit effects on photosynthesis, ribulose bisphosphate carboxylase-oxygenase, and carbohydrate metabolism in rice. Physiologia Plantarum 103, 327339.