The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner
Article first published online: 5 MAR 2002
The Plant Journal
Volume 3, Issue 4, pages 611–617, April 1993
How to Cite
Jonak, C., Páy, A., Börge, L., Hirt, H. and Heberle-Bors, E. (1993), The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner. The Plant Journal, 3: 611–617. doi: 10.1046/j.1365-313X.1993.03040611.x
- Issue published online: 5 MAR 2002
- Article first published online: 5 MAR 2002
- Received 29 July 1992; revised 18 September 1992; accepted 21 September 1992.
- Cited By
In animals, MAP kinase plays a key role in growth factor-stimulated signalling and in mitosis. The isolation of a Medicago sativa cDNA clone MsK7 which shows 52% identity to animal MAP kinases is reported. The deduced protein sequence shows all the important structural features of MAP kinases and also contains the highly conserved Thr-183 and Tyr-185 residues. Northern analysis of synchronized alfalfa cells showed that the MsK7 kinase gene is expressed at low levels in G1 phase but at higher levels in S and G2 phases of the cell cycle. In the plant, only stems and roots were found to contain MAP kinase MsK7 mRNA. Southern and PCR analyses indicated that alfalfa contains at least four highly related MAP kinase genes.