Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis
Article first published online: 5 MAR 2002
The Plant Journal
Volume 8, Issue 5, pages 659–671, November 1995
How to Cite
Shirley, B. W., Kubasek, W. L., Storz, G., Bruggemann, E., Koornneef, M., Ausubel, F. M. and Goodman, H. M. (1995), Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis. The Plant Journal, 8: 659–671. doi: 10.1046/j.1365-313X.1995.08050659.x
- Issue published online: 5 MAR 2002
- Article first published online: 5 MAR 2002
- Received 10 March 1995; revised 27 July 1995; accepted 24 August 1995.
- Cited By
Eleven loci that play a role in the synthesis of flavonoids in Arabidopsis are described. Mutations at these loci, collectively named transparent testa (tt), disrupt the synthesis of brown pigments in the seed coat (testa). Several of these loci (tt3, tt4, tt5 and ttg) are also required for the accumulation of purple anthocyanins in leaves and stems and one locus (ttg) plays additional roles in trichome and root hair development. Specific functions were previously assigned to tt1–7 and ttg. Here, the results of additional genetic, biochemical and molecular analyses of these mutants are described. Genetic map positions were determined for tt8, tt9 and tt10. Thin-layer chromatography identified tissue- and locus-specific differences in the flavonols and anthocyanidins synthesized by mutant and wild-type plants. It was found that UV light reveals distinct differences in the floral tissues of tt3, tt4, tt5, tt6 and ttg, even though these tissues are indistinguishable under visible light. Evidence was also uncovered that tt8 and ttg specifically affect dihydroflavonol reductase gene expression. A summary of these and previously published results are incorporated into an overview of the genetics of flavonoid biosynthesis in Arabidopsis.