Cell cycle-regulated histone gene expression in synchronized plant cells

Authors

  • Jean-Philippe Reichheld,

    1. Institut de Biologie Moléculaire des Plantes du C.N.R.S., Université Louis Pasteur, 12, rue du Général Zimmer, 67084 Strasbourg Cédex, France
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  • Seiji Sonobe,

    1. Faculty of Science, Himeji Institute of Technology, 1479-1 Kanaji Kamigoricho Akogun Hyogo 678-12, Japan
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  • Bernadette Clément,

    1. Institut de Biologie Moléculaire des Plantes du C.N.R.S., Université Louis Pasteur, 12, rue du Général Zimmer, 67084 Strasbourg Cédex, France
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  • Nicole Chaubet,

    1. Institut de Biologie Moléculaire des Plantes du C.N.R.S., Université Louis Pasteur, 12, rue du Général Zimmer, 67084 Strasbourg Cédex, France
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  • Claude Gigot

    Corresponding author
    1. Institut de Biologie Moléculaire des Plantes du C.N.R.S., Université Louis Pasteur, 12, rue du Général Zimmer, 67084 Strasbourg Cédex, France
      *For correspondence (fax +33 88 61 44 42).
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*For correspondence (fax +33 88 61 44 42).

Summary

The regulation of histone gene expression during the cell cycle has been studied in a synchronized tobacco (BY2) cell suspension. Maximal expression was found in S phase but unlike other eukaryotic organisms the amount of histone mRNA was not coupled to DNA synthesis. Using aphidicolin as an inhibitor of DNA synthesis either before or at mid-S phase an accumulation of histone mRNAs was found in the absence of any DNA synthesis which was not due to a stabilization of the mRNAs but to actual control at the transcriptional level. At the completion of unperturbed or delayed S phase, histone mRNAs were selectively degraded by a post-transcriptional mechanism requiring de novo transcription.

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