Both authors contributed equally to this work.
The CER3 gene of Arabidopsis thaliana is expressed in leaves, stems, roots, flowers and apical meristems
Article first published online: 5 MAR 2002
The Plant Journal
Volume 10, Issue 3, pages 459–467, September 1996
How to Cite
Hannoufa, A., Negruk, V., Eisner, G. and Lemieux, B. (1996), The CER3 gene of Arabidopsis thaliana is expressed in leaves, stems, roots, flowers and apical meristems. The Plant Journal, 10: 459–467. doi: 10.1046/j.1365-313X.1996.10030459.x
- Issue published online: 5 MAR 2002
- Article first published online: 5 MAR 2002
- Received 23 February 1996; revised 24 May 1996; accepted 21 June 1996.
- Cited By
The eceriferum3 (cer3) locus encodes one of 21 gene products known to be involved in wax biosynthesis in Arabidopsis thaliana. Mutations at these loci are readily identified by their bright, dark-green stems when compared with the more glaucous wild-type plant. Clones of a gene which encodes a 795 amino acid open reading frame have been isolated by using plant DNA flanking the site of a T-DNA insertion in line BRL1. Molecular complementation of the cer3 mutant phenotype by clones of this gene establish that it corresponds to the CER3 gene. Although the 90 kDa predicted amino acid sequence of this gene shows no homology to any other known protein, the second exon of CER3 encodes a RRX12KK nuclear localization sequence (NLS). Southern blot and DNA sequence analysis revealed that the T-DNA is inserted 89 bp downstream of the translation termination codon of this gene. Northern blot hybridization of RNA isolated from the BRL1 mutant with the CER3cDNA probe indicated that the transcript is absent in this mutant line. Unlike other CER genes that have been cloned to date, high levels of the CER3 transcript were found in all tissues from wild-type plants, that is, leaves, stems, roots, flowers, and apical meristems.