Structural instability of a transgene locus in tobacco is associated with aneuploidy
Article first published online: 5 MAR 2002
The Plant Journal
Volume 10, Issue 3, pages 469–478, September 1996
How to Cite
Papp, I., Iglesias, V.A., Moscone, E.A., Michalowski, S., Spiker, S., Park, Y.-D., Matzke, M.A. and Matzke, A.J.M. (1996), Structural instability of a transgene locus in tobacco is associated with aneuploidy. The Plant Journal, 10: 469–478. doi: 10.1046/j.1365-313X.1996.10030469.x
- Issue published online: 5 MAR 2002
- Article first published online: 5 MAR 2002
- Received 27 March 1996; revised 29 May 1996; accepted 28 June 1996.
- Cited By
This paper describes molecular and cytogenetic evidence for the instability of a transgene locus that is present on the triplicated chromosome in an aneuploid tobacco line. This instability was manifested in several ways in trisomics including a major chromosome rearrangement that was detectable cytogenetically, smaller scale DNA rearrangements that occurred both germinally and somatically, and methylation/epigenetic silencing. In a deletion derivative of the locus, DNA breakpoints were found in AT-rich regions. One of these regions binds to nuclear scaffolds in vitro, suggesting a possible role for aberrant topoisomerase II cleavage in destabilization of the locus. The implications of increased chromosome instability in aneuploids for plant karyotype evolution and human carcino-genesis are discussed.