Present address: Instituto Nacional de Ciencias Agricolas, Gaveta postal no.1, San Jose de las Layas, La Habana, C.P. 32700, Cuba.
Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci
Article first published online: 5 MAR 2002
The Plant Journal
Volume 10, Issue 4, pages 655–661, October 1996
How to Cite
Léon-Kloosterziel, K. M., Gil, M. A., Ruijs, G. J., Jacobsen, S. E., Olszewski, N. E., Schwartz, S. H., Zeevaart, J. A.D. and Koornneef, M. (1996), Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci. The Plant Journal, 10: 655–661. doi: 10.1046/j.1365-313X.1996.10040655.x
- Issue published online: 5 MAR 2002
- Article first published online: 5 MAR 2002
- Received 29 March 1996; revised 11 June 1996; accepted 15 July 1996.
- Cited By
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.