Identification of a salicylic acid-responsive element in the promoter of the tobacco pathogenesis-related β-1,3-glucanase gene, PR-2d
Article first published online: 31 JAN 2003
The Plant Journal
Volume 10, Issue 6, pages 1089–1101, December 1996
How to Cite
Shah, J. and Klessig, D. F. (1996), Identification of a salicylic acid-responsive element in the promoter of the tobacco pathogenesis-related β-1,3-glucanase gene, PR-2d. The Plant Journal, 10: 1089–1101. doi: 10.1046/j.1365-313X.1996.10061089.x
- Issue published online: 31 JAN 2003
- Article first published online: 31 JAN 2003
- Received 19 June 1996; accepted 5 September 1996.
- Cited By
The tobacco pathogenesis-related PR-2d gene encodes an acidic β-1,3-glucanase. Expression of the PR-2d:uidA(GUS) chimeric gene is induced in leaves undergoing the hyper-sensitive resistance response to tobacco mosaic virus and after treatment with salicylic acid (SA), a chemical believed to play an important role(s) in disease resistance. We have constructed transgenic tobacco plants which carry various segments of the PR-2d promoter fused to a heterologous core 35S promoter driving the uidA(GUS) reporter gene. Their analysis indicates that sequences from −364 to −288 upstream of the PR-2d transcription start site confer a high level of activation by SA (20-fold). Mutations within this sequence, located between −339 and −333, depressed SA activation. This region is also required for the SA-inducibility of a truncated PR-2d:GUS chimeric gene. Contained within this region is a 25 bp element located between −348 and −324 which was specifically recognized by nuclear factors from tobacco leaves. No conclusive differences were observed in the ability of proteins in nuclear extracts from water-treated versus SA-treated plants to bind to this cis element in vitro.