A transcriptional activation domain of ATMYB2, a drought-inducible Arabidopsis Myb-related protein

Authors

  • Takeshi Urao,

    1. Biological Resources Division, Japan International Research Center for Agricultural Science (JIRCAS), Ministry of Agriculture, Forestry and Fisheries, 1-2 Oowashi, Tsukuba, Ibaraki 305, Japan, and
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  • Masa-aki Noji,

    1. Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305, Japan
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    • Present address: Faculty of Pharmaceutical Sciences, Laboratory of Molecular Biology and Biotechnology Research Center of Medical Resources, Chiba University, Yayoi-cho 1-33, Inage-ku, Chiba 263, Japan.

  • Kazuko Yamaguchi-Shinozaki,

    1. Biological Resources Division, Japan International Research Center for Agricultural Science (JIRCAS), Ministry of Agriculture, Forestry and Fisheries, 1-2 Oowashi, Tsukuba, Ibaraki 305, Japan, and
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  • Kazuo Shinozaki

    Corresponding author
    1. Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305, Japan
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*For correspondence (fax +81 298 36 9060).

Summary

Trans-activation activity of ATMYB2, a drought-inducible Myb-related protein in Arabidopsis thaliana, was analyzed using a transient assay of Arabidopsis leaf protoplasts. ATMYB2 activated the transcription of a reporter gene from the MYB-binding site in a sequence-specific manner. Deletion of the C-terminal region of ATMYB2 reduced the trans-activation of the reporter gene, indicating that the acidic region at the C-terminus of ATMYB2 is required for transcriptional activation. The domain exchange analysis with the yeast GAL4 revealed that the C-terminal acidic region of ATMYB2 contains a sufficient domain for trans-activation. These results indicate that ATMYB2 acts as a transcriptional activator and that the C-terminal acidic region of ATMYB2 can function as a transcriptional activation domain.

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