Dissection of the ozone-induced calcium signature
Article first published online: 9 OCT 2008
The Plant Journal
Volume 17, Issue 5, pages 575–579, March 1999
How to Cite
Clayton, H., Knight, M. R., Knight, H., McAinsh, Martin R. and Hetherington, A. M. (1999), Dissection of the ozone-induced calcium signature. The Plant Journal, 17: 575–579. doi: 10.1046/j.1365-313X.1999.00411.x
- Issue published online: 9 OCT 2008
- Article first published online: 9 OCT 2008
- Received 9 October 1998; revised 20 January 1999; accepted 22 January 1999.
The cellular responses of plants to numerous extracellular stimuli are mediated by transient elevations in the concentration of cytosolic free calcium ([Ca2+]cyt). We have addressed the question of how cells can use this apparently ubiquitous system to initiate so many specific and appropriate end responses. We show that the pollutant gas ozone elicits a biphasic Ca2+ response in intact Arabidopsis plants and a subsequent increase in expression of the gene encoding the antioxidant defence enzyme glutathione-S-transferase (GST). The second of the two [Ca2+]cyt peaks, but not the first, could be eliminated either by pre-treatment of plants with lanthanum chloride, or by reducing the duration of ozone fumigation. Under these conditions, ozone-inducedGSTexpression was abolished. These data provide a functional dissection of the ozone Ca2+ signalling pathway and indicate that the second ozone-induced [Ca2+]cyt peak provides the necessary information to direct expression ofGST.