Arabidopsis thaliana proteins related to the yeast SIP and SNF4 interact with AKINα1, an SNF1-like protein kinase

Authors

  • Jean-Pierre Bouly,

    1. Laboratoire de Biologie du Développement des Plantes, Institut de Biotechnologie des Plantes, UMR CNRS 8618, Bâtiment 630, Université de Paris-Sud, F-91405 Orsay Cedex, France
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  • Lionel Gissot,

    1. Laboratoire de Biologie du Développement des Plantes, Institut de Biotechnologie des Plantes, UMR CNRS 8618, Bâtiment 630, Université de Paris-Sud, F-91405 Orsay Cedex, France
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  • Philippe Lessard,

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    • Present address: Département d’Ecophysiologie Végétale et de Microbiologie (DEVM), Bat 156, CEN Cadarache, F-13108 St Paul lez Durance Cedex 01, France.

  • Martin Kreis,

    1. Laboratoire de Biologie du Développement des Plantes, Institut de Biotechnologie des Plantes, UMR CNRS 8618, Bâtiment 630, Université de Paris-Sud, F-91405 Orsay Cedex, France
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  • Martine Thomas

    Corresponding authorSearch for more papers by this author

*For correspondence (fax +33 1 69 33 64 25;
e-mail martine.thomas @ibp.u-psud.fr).

Summary

AKINα1, a Ser/Thr kinase from Arabidopsis thaliana belongs to the highly conserved SNF1 family of protein kinases in eukaryotes. Recent data suggest that the plant SNF1-related kinases (SnRK1 family) are key enzymes implicated in the regulation of carbohydrate and lipid metabolism. In Saccharomyces cerevisiae and mammals, the SNF1 and AMPKα protein kinases interact with two other families of proteins, namely SNF4/AMPKγ and SIP1/SIP2/GAL83/AMPKβ, to form active heterotrimeric complexes. In this paper, we describe the characterisation of three novel cDNAs. AKINβ1 and AKINβ2 encode proteins similar to SIP1, SIP2 and GAL83 and AKINγ codes for a protein showing similarity with SNF4. Using the two-hybrid system, specific interactions have been shown between A. thaliana AKINβ1/β2, AKINγ and AKINγ as well as between the A. thaliana and S. cerevisiae subunits. Interestingly, AKINβ1, AKINβ2 and AKINγ mRNAs accumulate differentially in A. thaliana tissues and are modulated during development and under different growth conditions. These data suggest the presence in higher plants of a conserved heterotrimeric complex. Moreover, the differential transcription of different non-catalytic subunits can constitute a first level of regulation of the SNF1-like complex in plants.

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