Present address: Department of Biochemistry, Royal Holloway College, University of London, London TW20 OBE, UK.
Characterization of the unconventional myosin VIII in plant cells and its localization at the post-cytokinetic cell wall
Article first published online: 5 JAN 2002
The Plant Journal
Volume 19, Issue 5, pages 555–567, September
How to Cite
Reichelt, S., Knight, A. E., Hodge, T. P., Baluska, F., Samaj, J., Volkmann, D. and Kendrick-Jones, J. (1999), Characterization of the unconventional myosin VIII in plant cells and its localization at the post-cytokinetic cell wall. The Plant Journal, 19: 555–567. doi: 10.1046/j.1365-313X.1999.00553.x
- Issue published online: 5 JAN 2002
- Article first published online: 5 JAN 2002
- Received 19 May 1999; accepted 7 July 1999.
Myosins are a large superfamily of motor proteins which, in association with actin, are involved in intra- cellular motile processes. In addition to the conventional myosins involved in muscle contractility, there is, in animal cells, a wide range of unconventional myosins implicated in membrane-associated processes, such as vesicle transport and membrane dynamics. In plant cells, however, very little is known about myosins. We have raised an antibody to the recombinant tail region of Arabidopsis thaliana myosin 1 (a class VIII myosin) and used it in immunofluorescence and EM studies on root cells from cress and maize. The plant myosin VIII is found to be concentrated at newly formed cross walls at the stage in which the phragmoplast cytoskeleton has depolymerized and the new cell plate is beginning to mature. These walls are rich in plasmodesmata and we show that they are the regions where the longitudinal actin cables appear to attach. Myosin VIII appears to be localized in these plasmodesmata and we suggest that this protein is involved in maturation of the cell plate and the re-establishment of cytoplasmic actin cables at sites of intercellular communication.