The auxin signal for protoplast swelling is perceived by extracellular ABP1
Article first published online: 23 DEC 2001
The Plant Journal
Volume 27, Issue 6, pages 591–599, September 2001
How to Cite
Steffens, B., Feckler, C., Palme, K., Christian, M., Böttger, M. and Lüthen, H. (2001), The auxin signal for protoplast swelling is perceived by extracellular ABP1. The Plant Journal, 27: 591–599. doi: 10.1046/j.1365-313X.2001.01103.x
- Issue published online: 23 DEC 2001
- Article first published online: 23 DEC 2001
- Received 16 January 2001; revised 5 June 2001; accepted 8 June 2001.
- protoplast swelling response;
- signal transduction;
- antibody effects
Protoplasts of corn coleoptiles and Arabidopsis hypocotyls respond to the plant hormone auxin with a rapid change in volume. We checked the effect of antibodies directed against epitopes of auxin-binding protein 1 from Arabidopsis thaliana (AtERabp1) and Zea mays (ZmERabp1), respectively. Antibodies raised against the C-terminus of AtERabp1 inhibited the response to auxin, while antibodies raised against a part of box a, the putative auxin-binding domain, induced a swelling response similar to that caused by auxin treatment. Synthetic C-terminal oligopeptides of ZmERabp1 also caused a swelling response. These effects occurred regardless of whether the experiments were carried out with homologous (anti-AtERabp1 antibodies on Arabidopsis protoplasts or anti-ZmERabp1 antibodies in maize protoplasts) or heterologous immunological tools. The results indicate that the auxin signal for protoplast swelling is perceived by extracellular ABP1.